Sequence analysis on the mitochondrial COXI gene of recent clinical isolates of Plasmodium knowlesi in Klang valley, peninsular Malaysia

Fong, M.Y.; Lau, Y.L.; Chin, L.C.; Al-Mekhlafi, A.M.Q. (2011) Sequence analysis on the mitochondrial COXI gene of recent clinical isolates of Plasmodium knowlesi in Klang valley, peninsular Malaysia. Tropical Biomedicine, 28 (2). pp. 457-463. ISSN 0127-5720

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    Abstract

    The cytochrome oxidase subunit I (COXI) gene sequences of three recent (20072008) clinical Plasmodium knowlesi isolates from Klang Valley, peninsular Malaysia, were determined and compared with those of older (1960's) peninsular Malaysia, recent isolates from Sarawak (on Borneo Island), and an isolate from Thailand. Multiple alignment of the sequences showed that the three clinical isolates were more similar to the older peninsular Malaysia isolates than to those from Sarawak and Thailand. Phylogenetic tree based on the COXI sequences revealed three distinct clusters of P. knowlesi. The first cluster consisted of isolates from peninsular Malaysia, the second consisted of Sarawak isolates and the third composed of the Thailand isolate. The findings of this study highlight the usefulness of mitochondrial COXI gene as a suitable marker for phylogeographic studies of P. knowlesi.

    Item Type: Article
    Journal or Publication Title: Tropical Biomedicine
    Additional Information: Department of Parasitology, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia Corresponding author e-mail: fongmy@um.edu.my
    Uncontrolled Keywords: Parasitology
    Subjects: R Medicine
    Divisions: Faculty of Medicine
    Depositing User: Fong Mun Yik
    Date Deposited: 12 Jun 2012 09:56
    Last Modified: 12 Jun 2012 09:56
    URI: http://eprints.um.edu.my/id/eprint/3241

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