Autologous chondrocyte transplantation in the repair of full-thickness focal cartilage damage in rabbits

Kamarul, Tunku and Selvaratnam, L. and Masjuddin, T. and Ab-Rahim, S. and Ng, C. and Chan, K.Y. and Ahmad, T.S.T. (2008) Autologous chondrocyte transplantation in the repair of full-thickness focal cartilage damage in rabbits. Journal of Orthopaedic Surgery, 16 (2). pp. 230-236. ISSN 2309-4990, DOI https://doi.org/10.1177/230949900801600220.

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Official URL: https://doi.org/10.1177/230949900801600220

Abstract

PURPOSE:To compare the efficacy of autologous chondrocyte transplantation (ACT) versus non-operative measures for cartilage repair in rabbits. METHODS:Nine New Zealand white rabbits were used. Identical focal defects were created in the articular cartilage of both knees. One month later, the right knee was repaired via ACT, while the left knee was left untreated (control group). The quality of cartilage tissues in both knees was compared 3 months later, according to the quantitative analysis of glycosaminoglycan (GAG) in the cartilage and macroscopic examination of histology using the Brittberg/International Cartilage Research Society (ICRS) score. RESULTS:Microscopic examination showed enhanced regeneration following ACT repair. Quantification analysis revealed significantly higher cellular expression of GAG in the ACT-treated knees (1.12 vs 0.81 microgram GAGs/mg protein, p=0.008). The mean Brittberg/ICRS score was significantly higher in the treated knees (6.00 vs 1.89, p=0.007). CONCLUSION: ACT is superior to non-operative measures for repairing focal cartilage defects, as determined by favourable histological and immunohistological outcomes at the cellular level.

Item Type: Article
Funders: UNSPECIFIED
Additional Information: Tissue Engineering Group, Department of Orthopaedic Surgery, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia
Uncontrolled Keywords: Cartilage; Chondrocytes; Tissue Engineering; Transplantation, Autologous
Subjects: R Medicine
Divisions: Faculty of Medicine
Depositing User: Ms Haslinda Lahuddin
Date Deposited: 07 Jun 2012 06:46
Last Modified: 10 Dec 2020 03:46
URI: http://eprints.um.edu.my/id/eprint/3217

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