Human amniotic membrane as a chondrocyte carrier vehicle/substrate: in vitro study

Krishnamurithy, G.; Shilpa, P.N.; Ahmad, R.E.; Sulaiman, S.; Ng, C.L.L.; Kamarul , T. (2011) Human amniotic membrane as a chondrocyte carrier vehicle/substrate: in vitro study. Journal of Biomedical Materials Research Part A, 99A (3). pp. 500-506. ISSN 1549-3296

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    Abstract

    Human amniotic membrane (HAM) is an established biomaterial used in many clinical applications. However, its use for tissue engineering purposes has not been fully realized. A study was therefore conducted to evaluate the feasibility of using HAM as a chondrocyte substrate/carrier. HAMs were obtained from fresh human placenta and were process to produced air dried HAM (AdHAM) and freeze dried HAM (FdHAM). Rabbit chondrocytes were isolated and expanded in vitro and seeded onto these preparations. Cell proliferation, GAG expression and GAG/cell expression were measured at days 3, 6, 9, 12, 15, 21, and 28. These were compared to chondrocytes seeded onto plastic surfaces. Histological analysis and scanning electron microscopy was performed to observe cell attachment. There was significantly higher cell proliferation rates observed between AdHAM (13-51, P=0.001) or FdHAM (18-48, p=0.001) to chondrocytes in monolayer. Similarly, GAG and GAG/cell expressed in AdHAM (33-82, p=0.001; 22-60, p=0.001) or FdHAM (41-81, p=0.001: 28-60, p=0.001) were significantly higher than monolayer cultures. However, no significant differences were observed in the proliferation rates (p=0.576), GAG expression (p=0.476) and GAG/cell expression (p=0.135) between AdHAM and FdHAM. The histology and scanning electron microscopy assessments demonstrates good chondrocyte attachments on both HAMs. In conclusion, both AdHAM and FdHAM provide superior chondrocyte proliferation, GAG expression, and attachment than monolayer cultures making it a potential substrate/carrier for cell based cartilage therapy and transplantation. (C) 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 99A: 500-506, 2011.

    Item Type: Article
    Creators:
    1. Krishnamurithy, G.
    2. Shilpa, P.N.
    3. Ahmad, R.E.
    4. Sulaiman, S.
    5. Ng, C.L.L.
    6. Kamarul , T.(Department of Orthopaedic Surgery, Faculty of Medicine Building, University of Malaya, 50603 Kuala Lumpur, MALAYSIA)
    Journal or Publication Title: Journal of Biomedical Materials Research Part A
    Additional Information: Tissue Engineering Group, Department of Orthopaedic Surgery, NOCERAL, University of Malaya, 50603 Kuala Lumpur, Malaysia Department of Physiology, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia Department of Obstetric and Gynaecology, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia Department of Surgery, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia
    Uncontrolled Keywords: Human Amniotic Membrane; Articular Chondrocytes; Tissue Engineering; Orthopaedics; Biomaterials
    Subjects: R Medicine
    Divisions: Faculty of Medicine
    Depositing User: Ms Haslinda Lahuddin
    Date Deposited: 05 Jun 2012 09:51
    Last Modified: 11 Dec 2013 12:03
    URI: http://eprints.um.edu.my/id/eprint/3202

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