Enzymatic profiling of clinical and environmental isolates of Burkholderia pseudomallei.

Liew , S.M.; Tay , S.T.; Wongratanacheewin , S.; Puthucheary , S.D. (2012) Enzymatic profiling of clinical and environmental isolates of Burkholderia pseudomallei. Trop Biomed, 29 (1).

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Abstract

Melioidosis has been recognized as an important cause of sepsis in the tropics. The disease caused by an environmental saprophyte Burkholderia pseudomallei, affects mostly adults with underlying immunocompromised conditions. In this study, the enzymatic profiles of 91 clinical and 9 environmental isolates of B. pseudomallei were evaluated using the APIZYM system, in addition to assessment of protease, phospholipase C and sialidase activities using agar plate methods and other assays. The activity of 10 enzymes - alkaline phosphatase, esterase, esterase lipase, lipase, leucine arylamidase, valine arylamidase, cystine arylamidase, acid phosphatase, naphthol-AS-BI-phosphohydrolase and N-acetyl-β-glucosaminidase were detected in >75% of the clinical isolates. The majority of B. pseudomallei isolates in this study exhibited protease and phospholipase activities. No sialidase activity was detected. Five Burkholderia thailandensis isolates had similar APIZYM profiles as B. pseudomallei clinical isolates except for the lower detection rate for N-acetyl-β-glucosaminidase. The subtle differences in the number of enzymes secreted and the levels of enzymatic activities of phenotypically identical clinical and environmental strains of B. pseudomallei give weight to the fact that the causative agent of melioidodis originates from the environment.

Item Type: Article
Journal or Publication Title: Trop Biomed
Additional Information: Department of Medical Microbiology, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur Malaysia
Uncontrolled Keywords: Medical Microbiology
Subjects: R Medicine
Divisions: Faculty of Medicine
Depositing User: Mr. Faizal Hamzah
Date Deposited: 22 May 2012 12:26
Last Modified: 22 May 2012 12:26
URI: http://eprints.um.edu.my/id/eprint/3193

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