Plant regeneration and cellular behaviour studies in celosia cristata grown in vivo and in vitro

Taha , R.M.; Wafa , S.N. (2012) Plant regeneration and cellular behaviour studies in celosia cristata grown in vivo and in vitro. ScientificWorld Journal. ISSN 1537-744X

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Abstract

Tissue culture studies of Celosia cristata were established from various explants and the effects of various hormones on morphogenesis of this species were examined. It was found that complete plant regeneration occurred at highest percentage on MS medium supplemented with 2.0 mg/L NAA and 1.5 mg/L BAP, with the best response showed by shoot explants. In vitro flowering was observed on MS basal medium after six weeks. The occurrence of somaclonal variation and changes in cellular behavior from in vivo and in vitro grown plants were investigated through cytological studies and image analysis. It was observed that Mitotic Index (MI), mean chromosome numbers, and mean nuclear to cell area ratio of in vitro root meristem cells were slightly higher compared to in vivo values. However, in vitro plants produced lower mean cell areas but higher nuclear areas when compared to in vivo plants. Thus, no occurrence of somaclonal variation was detected, and this was supported by morphological features of the in vitro plants.

Item Type: Article
Creators:
  1. Taha , R.M.
  2. Wafa , S.N.
Journal or Publication Title: ScientificWorld Journal
Additional Information: Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia
Uncontrolled Keywords: Biological Sciences
Subjects: R Medicine
Divisions: Faculty of Medicine
Depositing User: Mr. Faizal Hamzah
Date Deposited: 22 May 2012 11:46
Last Modified: 22 May 2012 11:46
URI: http://eprints.um.edu.my/id/eprint/3190

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