The antiproliferative activity of Sclerotia of Lignosus rhinocerus (Tiger Milk Mushroom)

Lee, M.L.; Tan, N.H.; Fung, S.Y.; Tan, C.S.; Ng, S.T. (2012) The antiproliferative activity of Sclerotia of Lignosus rhinocerus (Tiger Milk Mushroom). Evidence-Based Complementary and Alternative Medicine, 2012. ISSN 1741-427X

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    Abstract

    Lignosus rhinocerus, the tiger milk mushroom, is one of the most important medicinal mushrooms used by the indigenous people of Southeast Asia and China. It has been used to treat breast cancer. A cold water extract (LR-CW) prepared from the sclerotia of L. rhinocerus cultivar was found to exhibit antiproliferative activity against human breast carcinoma (MCF-7) and human lung carcinoma (A549), with of 96.7 μg/mL and 466.7 μg/mL, respectively. In comparison, LR-CW did not show significant cytotoxicity against the two corresponding human normal cells, 184B5 (human breast cell) and NL 20 (human lung cell). DNA fragmentation studies suggested that the cytotoxic action of LR-CW against cancer cells is mediated by apoptosis. Sephadex G-50 gel filtration fractionation of LR-CW yielded a high-molecular-weight and a low-molecular-weight fraction. The high-molecular-weight fraction contains mainly carbohydrate (68.7%) and small amount of protein (3.6%), whereas the low-molecular-weight fraction contains 31% carbohydrate and was devoid of protein. Only the high-molecular-weight fraction exhibited antiproliferative activity against cancer cells, with of 70.0 μg/mL and 76.7 μg/mL, respectively. Thus, the cytotoxic action of the LR-CW is due to the high-molecular-weight fraction, either the proteins or protein-carbohydrate complex.

    Item Type: Article
    Creators:
    1. Lee, M.L.(CENAR and Department of Molecular Medicine, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia)
    2. Tan, N.H.(CENAR and Department of Molecular Medicine, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia)
    3. Fung, S.Y.(CENAR and Department of Molecular Medicine, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia)
    4. Tan, C.S.(Biotechnology Research Centre, MARDI, P.O. Box 12301, 50744 Kuala Lumpur, Malaysia)
    5. Ng, S.T.(Ligno Biotech Sdn Bhd, Taman Perindustrian Balakong Jaya 2, Selangor, 43300 Balakong Jaya, Malaysia)
    Journal or Publication Title: Evidence-Based Complementary and Alternative Medicine
    Additional Information: CENAR and Department of Molecular Medicine, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia
    Uncontrolled Keywords: Molecular Medicine; Tiger Milk Mushroom
    Subjects: R Medicine
    Divisions: Faculty of Medicine
    Depositing User: Mr. Faizal Hamzah
    Date Deposited: 17 May 2012 09:13
    Last Modified: 16 Dec 2014 12:56
    URI: http://eprints.um.edu.my/id/eprint/3182

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