Free radical scavenging, antimicrobial and immunomodulatory activities of Orthosiphon stamineus.

Alshawsh, M.A.; Abdulla, M.A.; Ismail, S.; Amin, Z.A.; Qader, S.W.; Hadi, H.A.; Harmal, N.S. (2012) Free radical scavenging, antimicrobial and immunomodulatory activities of Orthosiphon stamineus. Molecules, 17 (5). ISSN 1431-5157

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Abstract

<em>Orthosiphon stamineus </em>is considered an important traditional folk medicine. In this study ethanol and aqueous extracts of <em>O. stamineus</em> were evaluated<em> in vitro </em>for their antioxidant, antimicrobial as well as for their immunomodulatory properties on human peripheral blood mononuclear cells (PBMCs). The DPPH radical scavenging method was used for the determination of antioxidant activity, while the antibacterial efficacy was investigated by both disc diffusion method and Minimum Inhibitory Concentration (MIC) against four bacterial strains (Gram-positive and Gram-negative). Furthermore, the immunomodulatory potential of the extracts was investigated through the MTT assay. Aqueous extract <strong>of </strong><em>O. stamineus</em> exhibited significant free radical scavenging activity with IC<sub>50</sub> 9.6 µg/mL, whereas the IC<sub>50</sub> for the ethanol extract was 21.4 µg/mL. The best antimicrobial activity was shown by the aqueous extract of <em>O. stamineus </em>against <em>Staphylococcus aureus</em>, with inhibition zone of 10.5 mm and MIC value 1.56 mg/mL. Moreover, the results observed from the MTT assay showed that both plant extracts stimulated the PBMCs proliferation<em> in vitro</em> in a concentration-dependent manner, but the aqueous extract has remarkable activity against PBMCs. These findings indicate that O. stamineus</em> showed high antioxidant activity and may be considered as an immunomodulatory agent.

Item Type: Article
Creators:
  1. Alshawsh, M.A.
  2. Abdulla, M.A.
  3. Ismail, S.
  4. Amin, Z.A.
  5. Qader, S.W.
  6. Hadi, H.A.
  7. Harmal, N.S.
Journal or Publication Title: Molecules
Additional Information: Department of Molecular Medicine, Faculty of Medicine, University of Malaya, Kuala Lumpur 50603, Malaysia. alshaweshmam@yahoo.com
Uncontrolled Keywords: Molecular
Subjects: R Medicine
Divisions: UNSPECIFIED
Depositing User: Mr. Faizal Hamzah
Date Deposited: 11 May 2012 10:46
Last Modified: 15 Nov 2013 11:03
URI: http://eprints.um.edu.my/id/eprint/3147

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