Susceptibility of enterococcus faecalis biofilm to antibiotics and calcium hydroxide

Chai, W.L.; Hamimah, H.; Cheng Soo, C.; Sallam Atiya, A.; Abdullah, M. (2007) Susceptibility of enterococcus faecalis biofilm to antibiotics and calcium hydroxide. Journal of Oral Science, 49 (2). pp. 161-166. ISSN 1343-4934

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      Abstract

      The purpose of this study was to investigate the antimicrobial efficacy of six groups of antibiotics and calcium hydroxide against Enterococcus faecalis biofilm in a membrane filter model. Two-day-old E. faecalis (ATCC 29212) biofilm was exposed to ampicillin, co-trimoxazole, erythr omycin, oxytetracycline, vancomycin, vancomycin followed by gentamicin, Ca(OH)2, and phosphate-buffered saline (control). After 1 h of exposure, the antimicrobial activity was neutralized by washing each disc five times in PBS, and then the colony-forming units of the remaining viable bacteria on each disc were counted. The results revealed that only erythromycin, oxytetracycline and Ca(OH)2 showed 100 biofilm kill. An ANOVA with a Bonferroni post hoc test (P < 0.05) detected significant differences among the test agents, except in the ampicillin group versus the co-trimoxazole group. It is concluded that erythromycin, oxytetracycline and Ca(OH)2 are 100 effective in eliminating E. faecalis biofilm, whereas ampicillin, co-trimoxazole, vancomycin, and vancomycin followed by gentamicin are ineffective.

      Item Type: Article
      Creators:
      1. Chai, W.L.
      2. Hamimah, H.
      3. Cheng Soo, C.
      4. Sallam Atiya, A.
      5. Abdullah, M.
      Journal or Publication Title: Journal of Oral Science
      Additional Information: Journal of Oral Science
      Subjects: R Medicine > RK Dentistry
      Divisions: Faculty of Dentistry > Dept of General Dental Practice And Oral & Maxillofacial Imaging
      Depositing User: Associate Professor Dr. Wen Lin Chai
      Date Deposited: 08 May 2012 15:19
      Last Modified: 27 Oct 2014 15:22
      URI: http://eprints.um.edu.my/id/eprint/3049

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