Susceptibility of enterococcus faecalis biofilm to antibiotics and calcium hydroxide

Chai, W.L. and Hamimah, H. and Cheng Soo, C. and Sallam Atiya, A. and Abdullah, M. (2007) Susceptibility of enterococcus faecalis biofilm to antibiotics and calcium hydroxide. Journal of Oral Science, 49 (2). pp. 161-166. ISSN 1343-4934, DOI https://doi.org/10.2334/josnusd.49.161.

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Abstract

The purpose of this study was to investigate the antimicrobial efficacy of six groups of antibiotics and calcium hydroxide against Enterococcus faecalis biofilm in a membrane filter model. Two-day-old E. faecalis (ATCC 29212) biofilm was exposed to ampicillin, co-trimoxazole, erythr omycin, oxytetracycline, vancomycin, vancomycin followed by gentamicin, Ca(OH)2, and phosphate-buffered saline (control). After 1 h of exposure, the antimicrobial activity was neutralized by washing each disc five times in PBS, and then the colony-forming units of the remaining viable bacteria on each disc were counted. The results revealed that only erythromycin, oxytetracycline and Ca(OH)2 showed 100 biofilm kill. An ANOVA with a Bonferroni post hoc test (P < 0.05) detected significant differences among the test agents, except in the ampicillin group versus the co-trimoxazole group. It is concluded that erythromycin, oxytetracycline and Ca(OH)2 are 100 effective in eliminating E. faecalis biofilm, whereas ampicillin, co-trimoxazole, vancomycin, and vancomycin followed by gentamicin are ineffective.

Item Type: Article
Funders: UNSPECIFIED
Additional Information: Journal of Oral Science
Subjects: R Medicine > RK Dentistry
Divisions: Faculty of Dentistry > Dept of General Dental Practice And Oral & Maxillofacial Imaging
Depositing User: Associate Professor Dr. Wen Lin Chai
Date Deposited: 08 May 2012 07:19
Last Modified: 27 Oct 2014 07:22
URI: http://eprints.um.edu.my/id/eprint/3049

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