Development of a novel model for the investigation of implant-soft tissue interface

Chai, W.L.; Moharamzadeh, K.; Brook, I.M.; Emanuelsson, L.; Palmquist, A.; Van Noort, R. (2010) Development of a novel model for the investigation of implant-soft tissue interface. Journal of Periodontology, 81 (8). pp. 1187-1195. ISSN 0022-3492

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      Abstract

      Background: In dental implant treatment, the long-term prognosis is dependent on the biologic seal formed by the soft tissue around the implant. The in vitro investigation of the implant-soft tissue interface is usually carried out using a monolayer cell-culture model that lacks a polarized-cell phenotype. This study developed a tissue-engineered threedimensional oral mucosal model (3D OMM) to investigate the implant-soft tissue interface. Methods: A 3D OMM was constructed using primary human oral keratinocytes and fibroblasts cultured on a skin-derived scaffold at an air-liquid interface. A titanium implant was inserted into the engineered oral mucosa and further cultured to establish epithelial attachment. The 3D OMM was characterized using basic histology and immunostaining for cytokeratin (CK) 10 and CK13. Histomorphometric analyses of the implant-soft tissue interface were carried out using a light-microscopy (LM) examination of ground sections and semi-thin sections as well as scanning electron microscopy (SEM). Results: Immunohistochemistry analyses suggests that the engineered oral mucosa closely resembles the normal oral mucosa. The LM and SEM examinations reveal that the 3D OMM forms an epithelial attachment on the titanium surface. Conclusion: The 3D OMM provided mimicking peri-implant features as seen in an in vivo model and has the potential to be used as a relevant alternative model to assess implant-soft tissue interactions.

      Item Type: Article
      Creators:
      1. Chai, W.L.
      2. Moharamzadeh, K.
      3. Brook, I.M.
      4. Emanuelsson, L.
      5. Palmquist, A.
      6. Van Noort, R.
      Journal or Publication Title: Journal of Periodontology
      Additional Information: Cited By (since 1996): 2 Export Date: 5 April 2012 Source: Scopus CODEN: JOPRA PubMed ID: 20450401 Language of Original Document: English Correspondence Address: Chai, W. L.; Department of General Dental Practice and Oral and Maxillofacial Imaging, University of Malaya, Kuala Lumpur 50603, Malaysia; email: w.l.chai@sheffield.ac.uk Chemicals/CAS: titanium, 7440-32-6; Dental Implants; Dental Materials; Keratin-10, 147785-83-9; Keratin-13; Titanium, 7440-32-6 References: Brånemark, P.I., Hansson, B.O., Adell, R., Osseointegrated implants in the treatment of the edentulous jaw. Experience from a 10-year period (1977) Scand. J. Plast. Reconstr. Surg. 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      Uncontrolled Keywords: Cell Cultured techniques/methods Dental implants Epithelial attachment Immunohistochemistry Mouth mucosa/anatomy &histology Tissue engineering biomedical and dental materials cytokeratin 10 cytokeratin 13 tissue scaffold titanium adult article audiovisual equipment cell adhesion coculture culture technique cytology dental etching epithelium cell female fibroblast histology human keratinocyte mouth mucosa scanning electron microscopy skin surface property tooth brushing tooth implantation tooth prosthesis Cell Culture Techniques Coculture Techniques Dental Materials Dental Polishing Dental Prosthesis Design Epithelial Cells Fibroblasts Histocytological Preparation Techniques Humans Keratin-10 Keratin-13 Keratinocytes Microscopy, Electron, Scanning Models, Anatomic Surface Properties Tissue Scaffolds Young Adult
      Subjects: R Medicine > RK Dentistry
      Divisions: Faculty of Dentistry > Dept of General Dental Practice And Oral & Maxillofacial Imaging
      Depositing User: Associate Professor Dr. Wen Lin Chai
      Date Deposited: 08 May 2012 11:56
      Last Modified: 11 Dec 2013 11:56
      URI: http://eprints.um.edu.my/id/eprint/3041

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