Flavone Enhances Dengue Virus Type-2 (NGC Strain) infectivity and replication in vero cells

Zandi, K.; Lani, R.; Wong, P.F.; Teoh, B.-T.; Sam, S.-S.; Johari, J.; Mustafa, M.R.; AbuBakar, S. (2012) Flavone Enhances Dengue Virus Type-2 (NGC Strain) infectivity and replication in vero cells. Molecules, 17 (3). pp. 2437-2445. ISSN 1420-3049

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    Abstract

    This study investigates the effects of 2-phenyl-1-benzopyran-4-one (flavone) on DENV-2 infectivity in Vero cells. Virus adsorption and attachment and intracellular virus replication were investigated using a foci forming unit assay (FFUA) and quantitative RT-PCR, respectively. Addition of flavone (100 μg/mL) significantly increased the number of DENV-2 foci by 35.66 ± 1.52 and 49.66 ± 2.51 when added during and after virus adsorption to the Vero cells, respectively. The average foci size after 4 days of infection increased by 33 ± 2.11 and 89 ± 2.13. The DENV-2 specific RNA copy number in the flavone-treated infected cells increased by 6.41- and 23.1-fold when compared to the mock-treated infected cells. Flavone (100 μg/mL) did not promote or inhibit Vero cell proliferation. The CC50 value of flavone against Vero cells was 446 µg/mL. These results suggest that flavone might enhance dengue virus replication by acting antagonistically towards flavonoids known to inhibit dengue virus replication.

    Item Type: Article
    Creators:
    1. Zandi, K.
    2. Lani, R.
    3. Wong, P.F.
    4. Teoh, B.-T.
    5. Sam, S.-S.
    6. Johari, J.
    7. Mustafa, M.R.(Department of Pharmacology, Faculty of Medicine Building, University of Malaya, 50603 Kuala Lumpur, MALAYSIA)
    8. AbuBakar, S.
    Journal or Publication Title: Molecules
    Additional Information: Suggested Citation: Zandi K, Lani R, Wong P-F, Teoh B-T, Sam S-S, Johari J, et al. Flavone Enhances Dengue Virus Type-2 (NGC Strain) Infectivity and Replication in Vero Cells. Molecules. 2012;17(3):2437-45.
    Uncontrolled Keywords: Antiviral ; Dengue ; Flavones ; Flavonoid ; Molecule
    Subjects: R Medicine
    Divisions: Faculty of Medicine > School of Biological Sciences
    Depositing User: Ms Haslinda Lahuddin
    Date Deposited: 08 May 2012 09:35
    Last Modified: 12 Dec 2014 13:54
    URI: http://eprints.um.edu.my/id/eprint/3030

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