Flavone Enhances Dengue Virus Type-2 (NGC Strain) infectivity and replication in vero cells

Zandi, K.; Lani, R.; Wong, P.F.; Teoh, B.-T.; Sam, S.-S.; Johari, J.; Mustafa, M.R.; AbuBakar, S. (2012) Flavone Enhances Dengue Virus Type-2 (NGC Strain) infectivity and replication in vero cells. Molecules, 17 (3). pp. 2437-2445. ISSN 1420-3049

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    Abstract

    This study investigates the effects of 2-phenyl-1-benzopyran-4-one (flavone) on DENV-2 infectivity in Vero cells. Virus adsorption and attachment and intracellular virus replication were investigated using a foci forming unit assay (FFUA) and quantitative RT-PCR, respectively. Addition of flavone (100 μg/mL) significantly increased the number of DENV-2 foci by 35.66 ± 1.52 and 49.66 ± 2.51 when added during and after virus adsorption to the Vero cells, respectively. The average foci size after 4 days of infection increased by 33 ± 2.11 and 89 ± 2.13. The DENV-2 specific RNA copy number in the flavone-treated infected cells increased by 6.41- and 23.1-fold when compared to the mock-treated infected cells. Flavone (100 μg/mL) did not promote or inhibit Vero cell proliferation. The CC50 value of flavone against Vero cells was 446 µg/mL. These results suggest that flavone might enhance dengue virus replication by acting antagonistically towards flavonoids known to inhibit dengue virus replication.

    Item Type: Article
    Creators:
    1. Zandi, K.
    2. Lani, R.
    3. Wong, P.F.
    4. Teoh, B.-T.
    5. Sam, S.-S.
    6. Johari, J.
    7. Mustafa, M.R.(Department of Pharmacology, Faculty of Medicine Building, University of Malaya, 50603 Kuala Lumpur, MALAYSIA)
    8. AbuBakar, S.(Department of Medical Microbiology, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, MALAYSIA)
    Journal or Publication Title: Molecules
    Additional Information: Suggested Citation: Zandi K, Lani R, Wong P-F, Teoh B-T, Sam S-S, Johari J, et al. Flavone Enhances Dengue Virus Type-2 (NGC Strain) Infectivity and Replication in Vero Cells. Molecules. 2012;17(3):2437-45.
    Uncontrolled Keywords: Antiviral ; Dengue ; Flavones ; Flavonoid ; Molecule
    Subjects: R Medicine
    Divisions: Faculty of Medicine > School of Biological Sciences
    Depositing User: Ms Haslinda Lahuddin
    Date Deposited: 08 May 2012 09:35
    Last Modified: 07 Nov 2017 16:39
    URI: http://eprints.um.edu.my/id/eprint/3030

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