Xiong, Chuan and Li, Ping and Luo, Qiang and Phan, Chia Wei and Li, Qiang and Jin, Xin and Huang, Wenli (2021) Induction of apoptosis in Hela cells by a novel peptide from fruiting bodies of Morchella importuna via the mitochondrial apoptotic pathway. Evidence-Based Complementary and Alternative Medicine, 2021. ISSN 1741-427X, DOI https://doi.org/10.1155/2021/5563367.
Full text not available from this repository.Abstract
Morels (Morchella spp.) are a genus of edible fungi with important economic and medicinal value. In this study, a novel peptide (MIPP) was extracted from the fruiting bodies of Morchella importuna using gel filtration chromatography. Structural analysis showed that the molecular mass of MIPP is 831 Da, and it has a simple amino acid sequence: Ser-Leu-Ser-Leu-Ser-Val-Ala-Arg. To explore the antitumor activity of MIPP, the effect of MIPP on HeLa cell apoptosis and the underlying preventative mechanisms were investigated. Results showed that MIPP reduced the viability of HeLa cells in a concentration-dependent manner. TUNEL analysis and flow cytometric examination showed that MIPP decreased cell proliferation via a mitochondrial-dependent pathway, as manifested by downregulation of Bcl-2/Bax, promotion of the movement of cytochrome C from the mitochondria to the cytoplasm, and triggering of caspase-9 and caspase-3. Therefore, MIPP may be a promising tumor-preventive agent, especially in human cervical cancer.
| Item Type: | Article |
|---|---|
| Funders: | High-Tech Field Expansion Project of Sichuan Academy of Agricultural Sciences[2018GXTZ-001], Foundation for Subject Construction of Sichuan Academy of Agricultural Sciences[2021XKJS058], Sichuan Association for Science and Technology Young Scholar Project |
| Uncontrolled Keywords: | Cytochrome-C;Polysaccharide;Antioxidant;Antitumor;Activation;Caspase-3 |
| Subjects: | R Medicine R Medicine > RS Pharmacy and materia medica |
| Divisions: | Faculty of Pharmacy |
| Depositing User: | Ms Zaharah Ramly |
| Date Deposited: | 22 Aug 2022 02:59 |
| Last Modified: | 22 Aug 2022 02:59 |
| URI: | http://eprints.um.edu.my/id/eprint/28701 |
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