Subramaniam, Bavani and Arshad, Norhafiza M. and Malagobadan, Sharan and Misran, Misni and Nyamathulla, Shaik and Mun, Kein Seong and Nagoor, Noor Hasima (2021) Development and evaluation of 1 `-acetoxychavicol acetate (ACA)-loaded nanostructured lipid carriers for prostate cancer therapy. Pharmaceutics, 13 (4). ISSN 1999-4923, DOI https://doi.org/10.3390/pharmaceutics13040439.
Full text not available from this repository.Abstract
1 `-acetoxychavicol acetate (ACA) extracted from the rhizomes of Alpinia conchigera Griff (Zingiberaceae) has been shown to deregulate the NF- signaling pathway and induce apoptosis-mediated cell death in many cancer types. However, ACA is a hydrophobic ester, with poor solubility in an aqueous medium, limited bioavailability, and nonspecific targeting in vivo. To address these problems, ACA was encapsulated in a nanostructured lipid carrier (NLC) anchored with plerixafor octahydrochloride (AMD3100) to promote targeted delivery towards C-X-C chemokine receptor type 4 (CXCR4)-expressing prostate cancer cells. The NLC was prepared using the melt and high sheer homogenization method, and it exhibited ideal physico-chemical properties, successful encapsulation and modification, and sustained rate of drug release. Furthermore, it demonstrated time-based and improved cellular uptake, and improved cytotoxic and anti-metastatic properties on PC-3 cells in vitro. Additionally, the in vivo animal tumor model revealed significant anti-tumor efficacy and reduction in pro-tumorigenic markers in comparison to the placebo, without affecting the weight and physiological states of the nude mice. Overall, ACA-loaded NLC with AMD3100 surface modification was successfully prepared with evidence of substantial anti-cancer efficacy. These results suggest the potential use of AMD3100-modified NLCs as a targeting carrier for cytotoxic drugs towards CXCR4-expressing cancer cells.
| Item Type: | Article |
|---|---|
| Funders: | Universiti Malaya (RU030-2014C), Center for Research in Biotechnology for Agriculture (CEBAR) Research University grant (TU002F-2018) |
| Uncontrolled Keywords: | Nanostructured lipid carrier; 1′ -acetoxychavicol acetate; AMD3100; Targeted delivery; Prostate cancer |
| Subjects: | R Medicine > RM Therapeutics. Pharmacology T Technology > TA Engineering (General). Civil engineering (General) |
| Divisions: | Faculty of Science > Department of Chemistry |
| Depositing User: | Ms Zaharah Ramly |
| Date Deposited: | 29 Mar 2022 02:24 |
| Last Modified: | 29 Mar 2022 02:24 |
| URI: | http://eprints.um.edu.my/id/eprint/28614 |
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