Ngu, Ee-Ling and Ko, Chen-Lin and Tan, Cheng-Yau and Wong, Kah-Hui and Phang, Siew-Moi and Yow, Yoon-Yen (2021) Phytochemical profiling and in vitro screening for neuritogenic and antioxidant activities of spirulina platensis. Indian Journal of Pharmaceutical Education and Research, 55 (3). pp. 812-822. ISSN 0019-5464, DOI https://doi.org/10.5530/ijper.55.3.154.
Full text not available from this repository.Abstract
Background: In neurological diseases, neuronal loss is frequently associated with overproduction of free radicals and reduced level of endogenous neurotrophic factors. The blue-green microalga, Spirulina platensis is a well-known superfood with a high content of diverse nutrients and possesses several therapeutic properties. Here, we aimed to study the neuritogenic and antioxidant activities of Spirulina platensis UMACC 159. Materials and Methods: PC-12Adh (rat pheochromocytoma) cell was used to investigate the cytotoxicity effect of S. platensis UMACC 159 extracts (water, methanol, and ethanol) via 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Neuritogenic activity of the extracts towards PC-12Adh cell line was studied using neurite outgrowth assay and immunofluorescence imaging of neurofilaments. The extracts were screened for the phytochemical contents, and antioxidant activities using 2,2'-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), 2,2-Diphenyl-1-pircrylhydrazyl (DPPH) and reducing power. Results: Ethanol extract was found to exhibit the highest neuritogenic effect and enhanced the cytoskeleton formation in PC-12Adh cells at 6.25 mu g/mL. Ethanol extract also showed the highest total phenolic content (49.09 +/- 1.35 mg GAE/g), ABTS (EC50 of 1.34 +/- 0.01 mg/mL) and DPPH (EC50 of 0.45 +/- 0.04 mg/mL) scavenging activities (P <= 0.05), suggesting that the neuritogenic effect of ethanol extract was attributed to the phenolic compound(s) via antioxidant activity. Conclusion: Ethanol extract contains bioactive compound(s) with similar neuritogenic activity as nerve growth factor for neuronal survival, growth, and axonal regeneration. S. platensis has been proposed as a promising cognitive supplement.
| Item Type: | Article |
|---|---|
| Funders: | Sunway University |
| Uncontrolled Keywords: | Spirulina platensis; Antioxidants; Cytotoxicity; Neuronal outgrowth; Phytochemicals |
| Subjects: | R Medicine > R Medicine (General) |
| Divisions: | Faculty of Medicine > Anaesthesiology Department |
| Depositing User: | Ms Zaharah Ramly |
| Date Deposited: | 03 Aug 2022 03:50 |
| Last Modified: | 03 Aug 2022 03:50 |
| URI: | http://eprints.um.edu.my/id/eprint/28395 |
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