Chong, Zhuo Lin and Soe, Hui Jen and Ismail, Amni Adilah and Mahboob, Tooba and Chandramathi, Samudi and Sekaran, Shamala Devi (2021) Evaluation of the diagnostic accuracy of a new biosensors-based rapid diagnostic test for the point-of-care diagnosis of previous and recent dengue infections in Malaysia. Biosensors, 11 (5). ISSN 2079-6374, DOI https://doi.org/10.3390/bios11050129.
Full text not available from this repository.Abstract
Dengue is a major threat to public health globally. While point-of-care diagnosis of acute/recent dengue is available to reduce its mortality, a lack of rapid and accurate testing for the detection of previous dengue remains a hurdle in expanding dengue seroepidemiological surveys to inform its prevention, especially vaccination, to reduce dengue morbidity. This study evaluated ViroTrack Dengue Serostate, a biosensors-based semi-quantitative anti-dengue IgG (immunoglobulin G) immuno-magnetic agglutination assay for the diagnosis of previous and recent dengue in a single test. Blood samples were obtained from 484 healthy participants recruited randomly from two communities in Petaling district, Selangor, Malaysia. The reference tests were Panbio Dengue IgG indirect and capture enzyme-linked immunosorbent assays, in-house hemagglutination inhibition assay, and focus reduction neutralization test. Dengue Serostate had a sensitivity and specificity of 91.1% (95%CI 87.8-93.8) and 91.1% (95%CI 83.8-95.8) for the diagnosis of previous dengue, and 90.2% (95%CI 76.9-97.3) and 93.2% (95%CI 90.5-95.4) for the diagnosis of recent dengue, respectively. Its positive predictive value of 97.5% (95%CI 95.3-98.8) would prevent most dengue-naive individuals from being vaccinated. ViroTrack Dengue Serostate's good point-of-care diagnostic accuracy can ease the conduct of dengue serosurveys to inform dengue vaccination strategy and facilitate pre-vaccination screening to ensure safety.
| Item Type: | Article |
|---|---|
| Funders: | Malaysia Global Entrepreneurship Movement (GEM) (ColossusINNO2017), BluSense Diagnostics, Denmark |
| Uncontrolled Keywords: | Dengue; Biosensors; Immuno-magnetic agglutination assay; Point-of-care diagnosis; Rapid diagnostic test; Previous and recent dengue; Diagnostic accuracy; Seroepidemiological survey; vaccination strategy |
| Subjects: | Q Science > QD Chemistry |
| Divisions: | Faculty of Medicine |
| Depositing User: | Ms Zaharah Ramly |
| Date Deposited: | 08 Apr 2022 00:09 |
| Last Modified: | 08 Apr 2022 00:09 |
| URI: | http://eprints.um.edu.my/id/eprint/28323 |
Actions (login required)
 |
View Item |
