Ngoi, Soo Tein and Niek, Wen Kiong and Lee, Yee Wan and AbuBakar, Sazaly and Teh, Cindy Shuan Ju (2021) Genomic analysis revealed a novel genotype of methicillin-susceptible Staphylococcus aureus isolated from a fatal sepsis case in dengue patient. Scientific Reports, 11 (1). ISSN 2045-2322, DOI https://doi.org/10.1038/s41598-021-83661-8.
Full text not available from this repository.Abstract
Staphylococcus aureus (S. aureus) is an opportunistic pathogen capable of causing serious health implications in susceptible individuals once it invades the host's protective barriers. Methicillin-susceptible S. aureus (MSSA) often receives lesser attention although it has been frequently associated with serious infections in human. We aim to investigate the genomic features of a highly virulent yet pan susceptible MSSA strain (coded as HS-MSSA) which caused concurrent bacteraemia in a dengue patient, ultimately resulted in sepsis death of the patient. Whole genome sequence analysis was performed. The draft genome of HS-MSSA is approximately 2.78 Mb (GC content=32.7%) comprising of 2637 predicted coding sequences. In silico genotyping of the HS-MSSA strain revealed a novel combined genotype (t091/ST2990). The HS-MSSA carries a SaPIn1-like pathogenicity island that harbours the staphylococcal enterotoxin and enterotoxin-like genes (sec3 and selL). The strain-specific beta -lactamase (blaZ)-bearing plasmid region was identified in HS-MSSA. Core genome phylogeny showed that the HS-MSSA strain shared a common ancestry with the European MRSA clone. We report herein the genomic features of an MSSA lineage with novel genotype previously not reported elsewhere.
| Item Type: | Article |
|---|---|
| Funders: | University of Malaya Research Fund (RU008-2018) |
| Uncontrolled Keywords: | Genomic analysis; Staphylococcus aureus; Methicillin-susceptible; Fatal sepsis case; Dengue patient |
| Subjects: | R Medicine > R Medicine (General) |
| Divisions: | Faculty of Medicine Faculty of Medicine > Medicine Department Deputy Vice Chancellor (Research & Innovation) Office > Tropical Infectious Diseases Research and Education Centre |
| Depositing User: | Ms Zaharah Ramly |
| Date Deposited: | 17 Jun 2022 03:19 |
| Last Modified: | 17 Jun 2022 03:19 |
| URI: | http://eprints.um.edu.my/id/eprint/27940 |
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