Teoh, Hoe Leong and Aminudin, Norhaniza and Abdullah, Noorlidah (2021) In vitro antioxidation, antihepatic steatosis, and anti-inflammatory effects of ethyl acetate fraction from Auricularia nigricans (Agaricomycetes). International Journal of Medicinal Mushrooms, 23 (2). pp. 43-56. ISSN 1521-9437,
Full text not available from this repository.Abstract
Nonalcoholic fatty liver disease (NAFLD) is currently one of the most common liver diseases worldwide. Lifestyle modifications through the diet are the mainstay of treatment. Auricularia nigricans is a popular edible mushroom known to possess medicinal properties. Gas chromatography-mass spectrometry and liquid chromatography-tandem mass spectrometry analysis indicated that linoleic acid ethyl ester, butyl 9,12-octadecadienoate, 9,12-octadecadienoic acid, ergosta-5,7,22-trien-3-ol, 2(3,4-dihydroxyphenyl)-7-hydroxy-5-benzene propanoic acid, and 3,30-di-O-methyl ellagic acid were present in the A. nigricans ethyl acetate (EA) fraction. The cytotoxicity assay showed that the EA fraction was noncytotoxic to HepG2 cells at concentrations < 100 mu g/mL. In the antihepatic steatosis assay, 50 mu g/mL of EA fraction caused a decline in absorbance to 0.20 +/- 0.02 compared to palmitic acid (PA)-induced cells (0.24 +/- 0.02). Furthermore, cells treated with 50 mu g/mL and 25 mu g/mL of EA fraction contributed an approximately 1.12-fold and 1.08-fold decrease in lipid accumulation compared to PA-induced cells. Coincubation with PA and 25 mu g/mL of EA fraction decreased levels of tumor necrosis factor-alpha, interleukin (IL)-6, IL-8, and monocyte chemoattractant protein-1 to 140.48 +/- 8.12, 91.16 +/- 2.40, 184.00 +/- 22.68, and 935.88 +/- 39.36 pg/mL compared to PA-induced cells. The presence of the EA fraction also suppressed the stress-activated protein kinase/Jun amino-terminal kinase, p-38 mitogen-activated protein kinase, nuclear factor-kappa B, and signal transducer and activator of transcription 3 signaling pathways. In conclusion, these findings suggest that the A. nigricans EA fraction demonstrates antisteatotic effects involving antioxidant capacity, hypolipidemic effects, and anti-inflammatory capacity in the PA-induced NAFLD pathological cell model.
| Item Type: | Article |
|---|---|
| Funders: | Universiti Malaya (PG142-2012B), HIR-MOHE grant (F00000221001) |
| Uncontrolled Keywords: | Auricularia nigricans; Nonalcoholic fatty liver disease (NAFLD); Palmitic acid-induced HepG2 cells; Medicinal mushrooms |
| Subjects: | Q Science > Q Science (General) Q Science > QH Natural history > QH301 Biology |
| Divisions: | Faculty of Science > Institute of Biological Sciences |
| Depositing User: | Ms Zaharah Ramly |
| Date Deposited: | 14 Jun 2022 02:12 |
| Last Modified: | 14 Jun 2022 02:12 |
| URI: | http://eprints.um.edu.my/id/eprint/27617 |
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