Evaluation of Pichia pastoris-Expressed Recombinant Rhoptry Protein 2 of Toxoplasma gondii for Its Application in Diagnosis of Toxoplasmosis

Chang, P. Y.; Fong, M. Y.; Nissapatorn, V.; Lau, Y. L. (2011) Evaluation of Pichia pastoris-Expressed Recombinant Rhoptry Protein 2 of Toxoplasma gondii for Its Application in Diagnosis of Toxoplasmosis. American Journal of Tropical Medicine and Hygiene, 85 (3). pp. 485-489. ISSN 0002-9637

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Abstract

Rhoptry protein 2 (ROP2) of Toxoplasma gondii is a rhoptry-secreted protein that plays a critical role in parasitophorous vacuole membrane formation during invasion. In previous studies, ROP2 has been shown to be efficient in triggering humoral and cell-mediated responses. High immunogenicity of ROP2 makes it a potential candidate for diagnosis and vaccination against toxoplasmosis. In this study, the ROP2 gene was cloned into pPICZ alpha A expression vector and extracellularly expressed in the yeast Pichia pastoris, which has numerous advantages over other expression systems for eukaryotic proteins expression. The effectiveness of the secreted recombinant ROP2 as a diagnosis agent was assessed by Western Blot with 200 human serum samples. Recombinant ROP2 reacted with toxoplasmosis-positive human serum samples and yielded an overall sensitivity of 90 and specificity of 95. However, recombinant ROP2 is a better marker for detection of IgG (91.7) rather than IgM (80).

Item Type: Article
Creators:
  1. Chang, P. Y.
  2. Fong, M. Y.(Department of Parasitology, Faculty of Medicine Building, University of Malaya, 50603 Kuala Lumpur, MALAYSIA)
  3. Nissapatorn, V.
  4. Lau, Y. L.
Journal or Publication Title: American Journal of Tropical Medicine and Hygiene
Additional Information: Chang, Phooi Yee Fong, Mun Yik Nissapatorn, Veeranoot Lau, Yee Ling
Uncontrolled Keywords: Rhoptry protein 2 (ROP2)
Subjects: R Medicine
Divisions: Faculty of Medicine
Depositing User: Prof. Dr. Mun Yik Fong
Date Deposited: 22 Feb 2012 16:02
Last Modified: 22 Feb 2012 16:02
URI: http://eprints.um.edu.my/id/eprint/2743

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