Chang, P.Y. and Fong, M.Y. and Nissapatorn, V. and Lau, Y.L. (2011) Evaluation of Pichia pastoris-Expressed Recombinant Rhoptry Protein 2 of Toxoplasma gondii for Its Application in Diagnosis of Toxoplasmosis. American Journal of Tropical Medicine and Hygiene, 85 (3). pp. 485-489. ISSN 0002-9637, DOI https://doi.org/10.4269/ajtmh.2011.11-0351.
Full text not available from this repository.Abstract
Rhoptry protein 2 (ROP2) of Toxoplasma gondii is a rhoptry-secreted protein that plays a critical role in parasitophorous vacuole membrane formation during invasion. In previous studies, ROP2 has been shown to be efficient in triggering humoral and cell-mediated responses. High immunogenicity of ROP2 makes it a potential candidate for diagnosis and vaccination against toxoplasmosis. In this study, the ROP2 gene was cloned into pPICZ alpha A expression vector and extracellularly expressed in the yeast Pichia pastoris, which has numerous advantages over other expression systems for eukaryotic proteins expression. The effectiveness of the secreted recombinant ROP2 as a diagnosis agent was assessed by Western Blot with 200 human serum samples. Recombinant ROP2 reacted with toxoplasmosis-positive human serum samples and yielded an overall sensitivity of 90 and specificity of 95. However, recombinant ROP2 is a better marker for detection of IgG (91.7) rather than IgM (80).
| Item Type: | Article |
|---|---|
| Funders: | UNSPECIFIED |
| Additional Information: | Chang, Phooi Yee Fong, Mun Yik Nissapatorn, Veeranoot Lau, Yee Ling |
| Uncontrolled Keywords: | Rhoptry protein 2 (ROP2) |
| Subjects: | R Medicine |
| Divisions: | Faculty of Medicine |
| Depositing User: | Prof. Dr. Mun Yik Fong |
| Date Deposited: | 22 Feb 2012 08:02 |
| Last Modified: | 16 Dec 2014 04:21 |
| URI: | http://eprints.um.edu.my/id/eprint/2743 |
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