Genetic diversity and molecular identification of mosquito species in the Anopheles maculatus group using the ITS2 region of rDNA

Walton, C.; Somboon, P.; O'Loughlin, S.M.; Zhang, S.; Harbach, R.E.; Linton, Y.M.; Chen, B.; Nolan, K.; Duong, S.; Fong, M.Y.; Vythilingum, I.; Mohammed, Z.D.; Trung, H.D.; Butlin, R.K. (2007) Genetic diversity and molecular identification of mosquito species in the Anopheles maculatus group using the ITS2 region of rDNA. Infection Genetics and Evolution, 7 (1). pp. 93-102. ISSN 1567-1348

[img] PDF - Published Version
Restricted to Registered users only [error in script]

Download (516Kb) | Request a copy

    Abstract

    The species diversity and genetic structure of mosquitoes belonging to the Anopheles maculatus group in Southeast Asia were investigated using the internal transcribed spacer 2 (ITS2) of ribosomal DNA (rDNA). A molecular phylogeny indicates the presence of at least one hitherto unrecognised species. Mosquitoes of chromosomal form K from eastern Thailand have a unique ITS2 sequence that is 3.7 divergent from the next most closely related taxon (An. sawadwongporni) in the group. In the context of negligible intraspecific variation at ITS2, this suggests that chromosomal form K is most probably a distinct species. Although An. maculatus sensu stricto from northern Thailand and southern Thailand/peninsular Malaysia differ from each other in chromosomal banding pattern and vectorial capacity, no intraspecific variation was observed in the ITS2 sequences of this species over this entire geographic area despite an extensive survey. A PCR-based identification method was developed to distinguish five species of the group (An. maculatus, An. dravidicus, An. pseudowillmori, An. sawadwongporni and chromosomal form K) to assist field-based studies in northwestern Thailand. Sequences from 187 mosquitoes (mostly An. maculatus and An. sawadwongporni) revealed no intraspecific variation in specimens from Thailand, Cambodia, mainland China, Malaysia, Taiwan and Vietnam, suggesting that this identification method will be widely applicable in Southeast Asia. The lack of detectable genetic structure also suggests that populations of these species are either connected by gene flow and/or share a recent common history. (c) 2006 Elsevier B.V. All rights reserved.

    Item Type: Article
    Creators:
    1. Walton, C.
    2. Somboon, P.
    3. O'Loughlin, S.M.
    4. Zhang, S.
    5. Harbach, R.E.
    6. Linton, Y.M.
    7. Chen, B.
    8. Nolan, K.
    9. Duong, S.
    10. Fong, M.Y.(Department of Parasitology, Faculty of Medicine Building, University of Malaya, 50603 Kuala Lumpur, MALAYSIA)
    11. Vythilingum, I.
    12. Mohammed, Z.D.
    13. Trung, H.D.
    14. Butlin, R.K.
    Journal or Publication Title: Infection Genetics and Evolution
    Additional Information: Walton, C. Somboon, P. O'Loughlin, S. M. Zhang, S. Harbach, R. E. Linton, Y. -M. Chen, B. Nolan, K. Duong, S. Fong, M-Y. Vythilingum, I. Mohammed, Z. D. Trung, Ho Dinh Butlin, R. K.
    Uncontrolled Keywords: Anopheles maculatus group; Malaria; Southeast Asia; ITS2; Genetic structure
    Subjects: R Medicine
    Divisions: Faculty of Medicine
    Depositing User: Prof. Dr. Mun Yik Fong
    Date Deposited: 22 Feb 2012 15:58
    Last Modified: 11 Dec 2013 11:49
    URI: http://eprints.um.edu.my/id/eprint/2739

    Actions (For repository staff only: Login required)

    View Item

    Document Downloads

    More statistics for this item...