Sequence analysis of E/NS1 gene junction of dengue virus type 2 isolated in Brunei

Osman, O.; Fong, M.Y.; Devi, S. (2008) Sequence analysis of E/NS1 gene junction of dengue virus type 2 isolated in Brunei. Southeast Asian journal of tropical medicine and public health, 39 (1). pp. 62-78. ISSN 0125-1562

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    A preliminary study of dengue infection in Brunei between 2005 and 2006 showed that dengue 2 was the predominant serotype. A total of five DEN-2 isolates were isolated and maintained in the mosquito cell-line, albopictus C6/36. The sequence spanning the envelope and non-structural protein 1 (E/NS1) junction (positions 2311 to 2550) of the isolates were determined and analysed at the amino acid and nucleotide levels. Alignment of the 240 nucleotide sequences among the five isolates showed changes occurring at 7 positions (2.9) of the region. All but one nucleotide substitution (position 2319, amino acid 742 V�F) were found at the 3rd position of the codons and were silent mutations. Amino acid homology ranged from 98 to 100. Sequence divergence of the Brunei isolates varied from 5 to 6.6 compared with dengue-2 prototype New Guinea C strain. Comparison of the Brunei DEN-2 isolates with sixty-five other strains placed them in a cluster containing Indonesian strains isolated in 1973, 1978 and 2004 and Malaysian strains isolated in 1996, 1998 and 1999 in genotype group IV.

    Item Type: Article
    1. Osman, O.
    2. Fong, M.Y.(Department of Parasitology, Faculty of Medicine Building, University of Malaya, 50603 Kuala Lumpur, MALAYSIA)
    3. Devi, S.
    Journal or Publication Title: Southeast Asian journal of tropical medicine and public health
    Additional Information: Department of Medical Microbiology,Department of Parasitology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia
    Uncontrolled Keywords: Parasitology(Medical Parasitology),Diagnostics(Molecular Diagnosis and Recombinant Antigens),Virology(Molecular Characterisation and Epidemiology)
    Subjects: R Medicine
    Divisions: Faculty of Medicine
    Depositing User: Prof. Dr. Mun Yik Fong
    Date Deposited: 22 Feb 2012 15:31
    Last Modified: 21 Dec 2014 17:37

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