Complete genome sequence analysis of dengue virus type 2 isolated in Brunei

Osman, O.; Fong, M. Y.; Devi, S. (2008) Complete genome sequence analysis of dengue virus type 2 isolated in Brunei. Virus Research, 135 (1). pp. 48-52. ISSN 0168-1702

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    Abstract

    In a previous study, we have reported the detection and isolation of dengue virus in Brunei (Osman, O., Fong, M.Y., Devi, S., 2007. A preliminary study of dengue infection in Brunei. JJID 60 (4), 205-208). DEN-2 was the predominant serotype followed by DEN-1. The full genomic sequences of 3 DEN-2 viruses isolated during the 2005-2006 dengue incident in Brunei were determined. Twenty-five primer sets were designed to amplify contiguous overlapping fragments of approximately 500-600 base pairs spanning the entire sequence of the viral genome. The amplified PCR products were sent for sequencing and their nucleoticles and the deduced amino acids were determined. All three DEN-2 virus isolated were clustered in the Cosmopolitan genotype of the DEN-2 classification by Twiddy et al. This work constitutes the first complete genetic characterization of three Brunei DEN-2 virus strains. (c) 2008 Elsevier B.V. All rights reserved.

    Item Type: Article
    Journal or Publication Title: Virus Research
    Additional Information: Osman, Osmali Fong, Mun Yik Devi, Shamala
    Uncontrolled Keywords: Dengue Virus Type 2; Sequencing; Phylogenetic Analysis; Complete Genome; Brunei
    Subjects: R Medicine
    Divisions: Faculty of Medicine
    Depositing User: Prof. Dr. Mun Yik Fong
    Date Deposited: 22 Feb 2012 15:34
    Last Modified: 22 Feb 2012 15:34
    URI: http://eprints.um.edu.my/id/eprint/2734

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