Characterization of secreted recombinant Toxoplasma gondii surface antigen 2 (SAG2) heterologously expressed by the yeast Pichia pastoris

Fong, M. Y.; Lau, Y. L.; Zulqarnain, M. (2008) Characterization of secreted recombinant Toxoplasma gondii surface antigen 2 (SAG2) heterologously expressed by the yeast Pichia pastoris. Biotechnology letters, 30 (4). pp. 611-618. ISSN 0141-5492

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    Abstract

    The surface antigen 2 (SAG2) gene of the protozoan parasite, Toxoplasma gondii, was cloned and extracellularly expressed in the yeast Pichia pastoris. The effectiveness of the secreted recombinant SAG2 (rSAG2-S) as a serodiagnosis reagent was assessed by western blots and ELISA. In the western blot assay, rSAG2-S reacted with all Toxoplasma-antibody positive human serum samples but not with Toxoplasma-negative samples. In the ELISA, rSAG2-S yielded sensitivity rates ranging from 80 (IgG negative, IgM positive) to 100 (IgG positive, IgM negative). In vivo experiments showed that serum from mice immunized with rSAG2-S reacted specifically with the native SAG2 of T. gondii. These mice were protected when challenged with live cells of T. gondii.

    Item Type: Article
    Creators:
    1. Fong, M. Y.(Department of Parasitology, Faculty of Medicine Building, University of Malaya, 50603 Kuala Lumpur, MALAYSIA)
    2. Lau, Y. L.
    3. Zulqarnain, M.
    Journal or Publication Title: Biotechnology letters
    Additional Information: Fong, Mun Yik Lau, Yee Ling Zulqarnain, Mohamed
    Uncontrolled Keywords: Pichia Pastoris; Recombinant SAG2 Antigen; Toxoplasma Gondi
    Subjects: R Medicine
    Divisions: Faculty of Medicine
    Depositing User: Prof. Dr. Mun Yik Fong
    Date Deposited: 21 Feb 2012 11:46
    Last Modified: 21 Feb 2012 11:46
    URI: http://eprints.um.edu.my/id/eprint/2723

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