Characterization of secreted recombinant Toxoplasma gondii surface antigen 2 (SAG2) heterologously expressed by the yeast Pichia pastoris

Fong, M.Y.; Lau, Y.L.; Zulqarnain, M. (2008) Characterization of secreted recombinant Toxoplasma gondii surface antigen 2 (SAG2) heterologously expressed by the yeast Pichia pastoris. Biotechnology letters, 30 (4). pp. 611-618. ISSN 0141-5492

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    Abstract

    The surface antigen 2 (SAG2) gene of the protozoan parasite, Toxoplasma gondii, was cloned and extracellularly expressed in the yeast Pichia pastoris. The effectiveness of the secreted recombinant SAG2 (rSAG2-S) as a serodiagnosis reagent was assessed by western blots and ELISA. In the western blot assay, rSAG2-S reacted with all Toxoplasma-antibody positive human serum samples but not with Toxoplasma-negative samples. In the ELISA, rSAG2-S yielded sensitivity rates ranging from 80 (IgG negative, IgM positive) to 100 (IgG positive, IgM negative). In vivo experiments showed that serum from mice immunized with rSAG2-S reacted specifically with the native SAG2 of T. gondii. These mice were protected when challenged with live cells of T. gondii.

    Item Type: Article
    Creators:
    1. Fong, M.Y.(Department of Parasitology, Faculty of Medicine Building, University of Malaya, 50603 Kuala Lumpur, MALAYSIA)
    2. Lau, Y.L.(Department of Parasitology, Faculty of Medicine Building, University of Malaya, 50603 Kuala Lumpur, MALAYSIA)
    3. Zulqarnain, M.(Institute of Biological Science, Faculty of Science, University of Malaya, Kuala Lumpur, Malaysia)
    Journal or Publication Title: Biotechnology letters
    Additional Information: Fong, Mun Yik Lau, Yee Ling Zulqarnain, Mohamed
    Uncontrolled Keywords: Pichia Pastoris; Recombinant SAG2 Antigen; Toxoplasma Gondi
    Subjects: R Medicine
    Divisions: Faculty of Medicine
    Depositing User: Prof. Dr. Mun Yik Fong
    Date Deposited: 21 Feb 2012 11:46
    Last Modified: 11 Dec 2014 09:56
    URI: http://eprints.um.edu.my/id/eprint/2723

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