Sequence analysis on the mitochondrial COXI gene of recent clinical isolates of Plasmodium knowlesi in Klang valley, peninsular Malaysia

Fong, Mun Yik and Lau, Yee Ling and Chin, Lit Chien and Al-Mekhlafi, A.M.Q. (2011) Sequence analysis on the mitochondrial COXI gene of recent clinical isolates of Plasmodium knowlesi in Klang valley, peninsular Malaysia. Tropical Biomedicine, 28 (2). pp. 457-463. ISSN 0127-5720,

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Abstract

The cytochrome oxidase subunit I (COXI) gene sequences of three recent (20072008) clinical Plasmodium knowlesi isolates from Klang Valley, peninsular Malaysia, were determined and compared with those of older (1960's) peninsular Malaysia, recent isolates from Sarawak (on Borneo Island), and an isolate from Thailand. Multiple alignment of the sequences showed that the three clinical isolates were more similar to the older peninsular Malaysia isolates than to those from Sarawak and Thailand. Phylogenetic tree based on the COXI sequences revealed three distinct clusters of P. knowlesi. The first cluster consisted of isolates from peninsular Malaysia, the second consisted of Sarawak isolates and the third composed of the Thailand isolate. The findings of this study highlight the usefulness of mitochondrial COXI gene as a suitable marker for phylogeographic studies of P. knowlesi.

Item Type:	Article
Funders:	UNSPECIFIED
Additional Information:	Fong, Mun Yik Lau, Yee-Ling Chin, Lit-Chien Al-Mekhlafi, Abdulsalam M. Q.
Uncontrolled Keywords:	Parasitology (Medical Parasitology) Diagnostics (Molecular Diagnosis and Recombinant Antigens) Virology (Molecular Characterisation and Epidemiology)
Subjects:	R Medicine
Divisions:	Faculty of Medicine
Depositing User:	Prof. Dr. Mun Yik Fong
Date Deposited:	21 Feb 2012 03:44
Last Modified:	14 Nov 2019 07:58
URI:	http://eprints.um.edu.my/id/eprint/2722

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