Azrizal-Wahid, Noor and Azirun, Mohd Sofian and Low, Van Lun (2021) Flea-borne pathogens in the cat flea Ctenocephalides felis and their association with mtDNA diversity of the flea host. Comparative Immunology Microbiology and Infectious Diseases, 75. ISSN 0147-9571, DOI https://doi.org/10.1016/j.cimid.2021.101621.
Full text not available from this repository.Abstract
Flea-borne pathogens were screened from 100 individual cat fleas using a PCR approach, of which 38 % were infected with at least one bacterium. Overall, 28 % of the flea samples were positive for Bartonella as inferred from ITS DNA region. Of these, 25 % (7/28) were identified as Bartonella clarridgeiae, 42.9 % (12/28) as Bar-tonella henselae consisted of two different strains, and 32.1 % (9/28) as Bartonella koehlerae, which was detected for the first time in Malaysia. Sequencing of gltA amplicons detected Rickettsia DNA in 14 % of cat flea samples, all of them identified as Rickettsia asembonensis (100 %). None of the flea samples were positive for Mycoplasma DNA in 16S rRNA gene detection. Four fleas were co-infected with Bartonella and Rickettsia DNAs. Statistical analyses reveal no significant association between bacterial infection and mtDNA diversity of the cat flea. Nevertheless, in all types of pathogen infections, infected populations demonstrated lower nucleotide and haplotype diversities compared to uninfected populations. Moreover, lower haplotype numbers were observed in infected populations.
| Item Type: | Article |
|---|---|
| Funders: | Universiti Malaya [RP021C-16SUS], Higher Institution Centre of Excellence (HICoE) program [MO002-2019] |
| Uncontrolled Keywords: | Flea; Ctenocephalides felis; Pathogens; Association; Genetic diversity |
| Subjects: | Q Science > QR Microbiology > QR180 Immunology S Agriculture > SF Animal culture |
| Divisions: | Faculty of Science > Institute of Biological Sciences Deputy Vice Chancellor (Research & Innovation) Office > Tropical Infectious Diseases Research and Education Centre |
| Depositing User: | Ms Zaharah Ramly |
| Date Deposited: | 13 Apr 2022 08:02 |
| Last Modified: | 13 Apr 2022 08:02 |
| URI: | http://eprints.um.edu.my/id/eprint/27039 |
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