Lai, Meng Yee and Tang, Soo Nee and Lau, Yee Ling (2021) Colorimetric reverse transcription-loop-mediated isothermal amplification assay for rapid detection of SARS-CoV-2. American Journal of Tropical Medicine and Hygiene, 105 (2). pp. 375-377. ISSN 0002-9637, DOI https://doi.org/10.4269/ajtmh.21-0150.
Full text not available from this repository.Abstract
Coronavirus disease 2019 (COVID-19), which is caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has been spreading rapidly all over the world. In the absence of effective treatments or a vaccine, there is an urgent need to develop a more rapid and simple detection technology of COVID-19. We describe a WarmStart colorimetric reverse transcription-loop-mediated isothermal amplification (RT-LAMP) assay for the detection of SARSCoV-2. The detection limit for this assay was 1 copy/mu L SARS-CoV-2. To test the clinical sensitivity and specificity of the assay, 37 positive and 20 negative samples were used. The WarmStart colorimetric RT-LAMP had 100% sensitivity and specificity. End products were detected by direct observation, thereby eliminating the need for post-amplification processing steps. WarmStart colorimetric RT-LAMP provides an opportunity to facilitate virus detection in resource limited settings without a sophisticated diagnostic infrastructure.
| Item Type: | Article |
|---|---|
| Funders: | Prototype Research Grant Scheme [PR001-2020B], Ministry of Education, Malaysia |
| Uncontrolled Keywords: | Coronavirus disease 2019 (COVID-19); Acute respiratory syndrome coronavirus 2 (SARS-CoV-2); Virus detection |
| Subjects: | R Medicine > RA Public aspects of medicine |
| Divisions: | Faculty of Medicine |
| Depositing User: | Ms Zaharah Ramly |
| Date Deposited: | 04 Apr 2022 06:43 |
| Last Modified: | 04 Apr 2022 06:43 |
| URI: | http://eprints.um.edu.my/id/eprint/27018 |
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