Ong, Chong-Boon and Annuar, Mohamad Suffian Mohamad (2021) Cross-linked tannase-carbon nanotubes composite in elevating antioxidative potential of green tea extract. Journal of Food Biochemistry, 45 (10). ISSN 0145-8884, DOI https://doi.org/10.1111/jfbc.13924.
Full text not available from this repository.Abstract
Multi-walled carbon nanotubes (MWCNT)-tannase composite was investigated as an immobilized biocatalyst on the basis of its facile preparation, low cost, and excellent aqueous dispersibility. Cross-linked tannase enzymes, obtained in the presence of glutaraldehyde, were composited with MWCNT via physical adsorption. Multiple techniques were applied to investigate, and corroborate the successful adsorption of cross-linked tannase onto the MWCNT structure. Green tea infusion extract post-treatment using the composite preparation showed elevated radical scavenging activities relative to the control. Green tea infusion extract exhibited a markedly reduced EC50 value on 2,2-diphenyl-1-picrylhydrazyl (DPPH) radicals following its treatment with the enzyme composite, which represents 20%-34% enhancement in its free radical scavenging capacity. Stoichiometry and number of reduced DPPH were determined and compared. The antioxidative potential of a widely consumed, health-beneficial green tea is elevated by the treatment with MWCNT-tannase composite. Practical applications Cross-linked tannase enzymes were composited with pristine multi-walled carbon nanotubes via simple physical adsorption. The composite presents key advantages such as low specific volume compared to other well-known immobilization media, inert, facile enzyme composition, and ease of recovery for repeated use. The work demonstrated carbon nanotube prosthetic utility in the biotransformation of food-based health commodity sought after for its nutritional benefits. The approach is of both industrial- and agricultural importance, and is a promising and viable strategy to obtain a natural, functional food supplement for the multi-billion dollar well-being and health-related industries.
| Item Type: | Article |
|---|---|
| Funders: | Universiti Malaya [PG033--2013A], International University of Malaya--Wales [R0007] |
| Uncontrolled Keywords: | Antioxidants; Composite; Green tea; Immobilization; Multi-walled carbon nanotubes; Tannase |
| Subjects: | Q Science > QH Natural history > QH301 Biology |
| Divisions: | Faculty of Science |
| Depositing User: | Ms Zaharah Ramly |
| Date Deposited: | 11 Apr 2022 07:25 |
| Last Modified: | 12 Apr 2022 00:47 |
| URI: | http://eprints.um.edu.my/id/eprint/26921 |
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