Ong, Agnes Lee Chen and Lee, Sau Har and Aung, Shuh Wen and Khaing, Si Lay and Ramasamy, Thamil Selvee (2021) 5-Azacytidine pretreatment confers transient upregulation of proliferation and stemness in human mesenchymal stem cells. Cells & Development, 165. ISSN 2667-2901,
Full text not available from this repository.Abstract
Successful outcomes of cell-based therapeutic is highly-dependent on quality and quantity of the cells. Epigenetic modifiers are known to modulate cell fates via reprogramming, hence it is plausible to use them in enhancing the plasticity of mesenchymal stem cells. In this study, we aimed to study the effects of 5-Azacytidine (5-AzaCR), an epigenetic modifier, pretreatment on mesenchymal stem cells-derived from Wharton's Jelly (WJMSCs) fates. WJMSCs were pretreated with 5-AzaCR for 24 h and subsequently cultured in culture media mixtures. The proliferative and stemness characteristics of the pretreated WJMSCs were assessed through morphological and gene expression analyses. Results showed that cells pretreated with 5 mu M to 20 mu M of 5-AzaCR showed to acquire higher proliferative state transiently when cultured in embryonic-mesenchymal stem cell (ESC-MSC) media, but not in MSC medium alone, and this coincides with significant transitional upregulation of stemness transcription factors. 5-AzaCR pretreatment has potential to confer initial induction of higher state of stemness and proliferation in WJMSCs, influenced by the culture media.
| Item Type: | Article |
|---|---|
| Funders: | Universiti Malaya, Malaysia through UMRG Programme Grant (RP019C-13HTM), Ministry of Education, Malaysia Ministry of Education, through Fundamental Research Grant Scheme (FP044-2014B/FRGS/2/2014/SKK01/UM/02/6) |
| Uncontrolled Keywords: | Epigenetic modifier; Stem cell therapy; Reprogramming; Proliferation; Stemness |
| Subjects: | Q Science > QR Microbiology |
| Divisions: | Faculty of Medicine |
| Depositing User: | Ms. Juhaida Abd Rahim |
| Date Deposited: | 23 Feb 2022 05:53 |
| Last Modified: | 23 Feb 2022 05:53 |
| URI: | http://eprints.um.edu.my/id/eprint/26836 |
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