Tan, Yong-Hui and Lim, Crystale Siew-Ying and Wong, Kah-Hui and Sabaratnam, Vikinewwary (2021) Neuritin protein expression is positively correlated with neurite outgrowth induced by the tiger milk mushroom, lignosus rhinocerotis (Agaricomycetes), in PC12 cells. International Journal of Medicinal Mushrooms, 23 (6). pp. 1-11. ISSN 1521-9437,
Full text not available from this repository.Abstract
: Neuritin is important in neuritogenesis, neurite arborization, and neurite extension. Lignosus rhinocerotis sclerotia extracts and nerve growth factor (NGF) have been well documented to possess positive neurite stimulatory effects. However, the correlation of neuritin expression with neurite outgrowth of L. rhinocerotis and NGF cotreatment of PC12 cells remains unknown. Thus, the present study investigated neuritin expression in PC12 cells treated with 5 ng/mL of NGF and L. rhinocerotis extracts (20-1280 mu g/mL) concurrently for 48 h. The neurite outgrowth score was quantitated, and total protein was harvested for enzyme-linked immunosorbent assay. There was a significant difference (P = 0.051) in neuritin protein abundance in 640 mu g/mL of L. rhinocerotis aqueous cotreatment with 5 ng/mL of NGF-treated cells (5 +/- 0.39 ng/mL) and 50 ng/mL of NGF-treated PC12 cells (5 +/- 0.48 ng/mL) compared to untreated cells (1.9 +/- 0.65 ng/ mL), with an average neurite length of 98 +/- 3.66, 106 +/- 3.00, and 73 +/- 4.79 mu m, respectively. Expression of microtubule element beta 3 tubulin was increased in PC12 cells treated with 50 ng/mL of NGF (3.5 +/- 0.21-fold) and also cells cotreated with 640 mu g/mL of extract and 5 ng/mL of NGF (4.9 +/- 0.29-fold) compared to untreated cells. Upregulation of beta 3 tubulin expression in this study confirmed the elongation of PC12 cell processes. Correlation analysis showed that neuritin protein abundance is positively proportional to the average neurite length in PC12 cells cotreated with L. rhinocerotis extract and 5 ng/mL of NGF. This study highlights that neuritin modulation is involved in neurite outgrowth induced by L. rhinocerotis treatment. To our knowledge, this is the first report to show that tiger milk mushroom extracts induce neuritin expression.
| Item Type: | Article |
|---|---|
| Funders: | Universiti Malaya (PG153-2014B), UCSI University Pioneer Scientists Incentive Fund (PSIF) (Proj-In-FAS-043), Ministry of Education, Malaysia (UM-MOHE UM.C/625/1/HIR/MOHE/ASG/01) |
| Uncontrolled Keywords: | Beta 3 tubulin; Lignosus rhinocerotis; Medicinal mushrooms; Nerve growth factor; Neurite stimulatory effect; Neuritin; Pheochromocytoma cells |
| Subjects: | Q Science > QH Natural history Q Science > QH Natural history > QH301 Biology |
| Divisions: | Faculty of Science > Institute of Biological Sciences |
| Depositing User: | Ms. Juhaida Abd Rahim |
| Date Deposited: | 28 Mar 2022 03:46 |
| Last Modified: | 28 Mar 2022 03:46 |
| URI: | http://eprints.um.edu.my/id/eprint/26599 |
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