Akinsola, Rasaq Olajide and Lee, Choon Weng and Sim, Edmund Ui Hang and Narayanan, Kumaran (2021) Inhibition of lysosomal vacuolar proton pump down-regulates cellular acidification and enhances E. coli bactofection efficiency. Analytical Biochemistry, 616. ISSN 0003-2697, DOI https://doi.org/10.1016/j.ab.2020.114088.
Full text not available from this repository.Abstract
Endosomal escape is considered a crucial barrier that needs to be overcome by integrin-mediated E. coli for gene delivery into mammalian cells. Bafilomycin, a potent inhibitor of the H+ proton pump commonly employed to lower endosomal pH, was evaluated as part of the E. coli protocol during delivery. We found an increase in green fluorescent protein expression up 6.9, 3.2, 5.0, 2.8, and 4.5 fold in HeLa, HEK-293, A549, HT1080, and MCF-7 respectively, compared to untreated cells. Our result showed for the first time that Inhibition of lysosomal V-ATPase enhances E. coli efficiency.
| Item Type: | Article |
|---|---|
| Funders: | MOSTI eScience grant from the Ministry of Science, Technology, and Innovation, Malaysia (02-02-10-SF0252), Monash University |
| Uncontrolled Keywords: | Proton pump inhibitor; Endosome; Bacteria DNA delivery; Lysosome; V-ATPase; Endocytosed |
| Subjects: | Q Science > QH Natural history > QH301 Biology |
| Divisions: | Faculty of Science > Institute of Biological Sciences |
| Depositing User: | Ms. Juhaida Abd Rahim |
| Date Deposited: | 15 Mar 2022 08:21 |
| Last Modified: | 15 Mar 2022 08:21 |
| URI: | http://eprints.um.edu.my/id/eprint/26536 |
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