Karlson, Chou Khai Soong and Mohd-Noor, Siti Nurfadhlina and Nolte, Nadja and Tan, Boon Chin (2021) CRISPR/dCas9-based systems: Mechanisms and applications in plant sciences. Plants-Basel, 10 (10). ISSN 2223-7747, DOI https://doi.org/10.3390/plants10102055.
Full text not available from this repository.Abstract
RNA-guided genomic transcriptional regulation tools, namely clustered regularly interspaced short palindromic repeats interference (CRISPRi) and CRISPR-mediated gene activation (CRISPRa), are a powerful technology for gene functional studies. Deriving from the CRISPR/Cas9 system, both systems consist of a catalytically dead Cas9 (dCas9), a transcriptional effector and a single guide RNA (sgRNA). This type of dCas9 is incapable to cleave DNA but retains its ability to specifically bind to DNA. The binding of the dCas9/sgRNA complex to a target gene results in transcriptional interference. The CRISPR/dCas9 system has been explored as a tool for transcriptional modulation and genome imaging. Despite its potential applications and benefits, the challenges and limitations faced by the CRISPR/dCas9 system include the off-target effects, protospacer adjacent motif (PAM) sequence requirements, efficient delivery methods and the CRISPR/dCas9-interfered crops being labeled as genetically modified organisms in several countries. This review highlights the progression of CRISPR/dCas9 technology as well as its applications and potential challenges in crop improvement.</p>
| Item Type: | Article |
|---|---|
| Funders: | UNSPECIFIED |
| Uncontrolled Keywords: | CRISPR interference; Crop improvement; Gene silencing; RNAi; Transcriptional regulation; CRISPR/dCas9 system |
| Subjects: | Q Science > Q Science (General) |
| Divisions: | Deputy Vice Chancellor (Research & Innovation) Office > Centre for Research in Biotechnology for Agriculture |
| Depositing User: | Ms. Juhaida Abd Rahim |
| Date Deposited: | 28 Feb 2022 01:26 |
| Last Modified: | 28 Feb 2022 01:26 |
| URI: | http://eprints.um.edu.my/id/eprint/26413 |
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