Detection and confirmation of dengue pre‐ and postintroduction of dengue NS1‐antigen test at the University Malaya Medical Centre: An observational study

Abd Jamil, Juraina and Azizan, Noor Syahida and Che Mat Seri, Nurul Asma Anati and Yaacob, Che Norainon and Samsudin, Nur Izyan and Mahfodz, Nur Hidayana and Zulkifli, Mulya Mustika Sari and Poh, Hooi Sim and Teoh, Boon Teong and AbuBakar, Sazaly (2021) Detection and confirmation of dengue pre‐ and postintroduction of dengue NS1‐antigen test at the University Malaya Medical Centre: An observational study. Journal of Medical Virology, 93 (8). pp. 4714-4719. ISSN 0146-6615

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Official URL: https://doi.org/10.1002/jmv.26790

Abstract

Early diagnosis of dengue is important to ensure proper management of patients and effective implementation of control measures. The present study was undertaken to determine the outcome of the implementation of dengue NS1-antigen (Ag) rapid diagnostic test (RDT) in the confirmation of dengue at the first patient hospital visit at the University Malaya Medical Centre. A total of 1036 and 1097 sera from the year 2008 and 2015 were used, representing samples from before and after dengue NS1-Ag RDT was implemented as routine diagnostic at the hospital. Results showed that similar dengue confirmation percentage (56%) was made in 2008 and 2015, regardless of the main laboratory diagnostic method used. Confirmation of dengue, however, increased to 68% and 73% when dengue NS1-Ag test or dengue immunoglobulin M-capture enzyme-linked immunosorbent assay was used as the second test for the 2008 and 2015 samples, respectively. Detection of dengue virus (DENV) using multiplex reverse transcription-polymerase chain reaction (RT-PCR) showed that DENV-1 was the highest in circulation in 2008 and that both DENV-1 and DENV-2 were dominant in 2015. In summary, the present study demonstrated that the introduction and use of the dengue NS1-Ag RDT did not change or compromise confirmation of dengue, highlighting the advantage of using the method. With the reducing cost of molecular detection tools, DENV detection using RT-PCR remains a viable option for further confirmation of dengue in hospital settings. © 2021 Wiley Periodicals LLC

Item Type: Article
Uncontrolled Keywords: dengue; diagnostics; infectious disease; tropical; vector-borne
Subjects: R Medicine
Divisions: Deputy Vice Chancellor (Research & Innovation) Office > Tropical Infectious Diseases Research and Education Centre
Depositing User: Ms. Juhaida Abd Rahim
Date Deposited: 26 Jul 2021 04:49
Last Modified: 26 Jul 2021 04:49
URI: http://eprints.um.edu.my/id/eprint/25993

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