Identification of potential glycoprotein biomarkers in oral squamous cell carcinoma using sweet strategies

Wong, Yin Ling and Anand, Ramanathan and Yuen, Kar Mun and Mustafa, Wan Mahadzir Wan and Abraham, Mannil Thomas and Tay, Keng Kiong and Rahman, Zainal Ariff Abdul and Chen, Yeng (2021) Identification of potential glycoprotein biomarkers in oral squamous cell carcinoma using sweet strategies. Glycoconjugate Journal, 38 (1). pp. 1-11. ISSN 0282-0080

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Official URL: https://doi.org/10.1007/s10719-021-09973-z

Abstract

The prevalence of oral squamous cell carcinoma (OSCC) is high in South and Southeast Asia regions. Most OSCC patients are detected at advanced stages low 5-year survival rates. Aberrant expression of glycosylated proteins was found to be associated with malignant transformation and cancer progression. Hence, identification of cancer-associated glycoproteins could be used as potential biomarkers that are beneficial for diagnosis or clinical management of patients. This study aims to identify the differentially expressed glycoproteins using lectin-based glycoproteomics approaches. Serum samples of 40 patients with OSCC, 10 patients with oral potentially malignant disorder (OPMD), and 10 healthy individuals as control group were subjected to two-dimensional gel electrophoresis (2-DE) coupled with lectin Concanavalin A and Jacalin that specifically bind to N- and O-glycosylated proteins, respectively. Five differentially expressed N- and O-glycoproteins with various potential glycosylation sites were identified, namely N-glycosylated α1-antitrypsin (AAT), α2-HS-glycoprotein (AHSG), apolipoprotein A-I (APOA1), and haptoglobin (HP); as well as O-glycosylated AHSG and clusterin (CLU). Among them, AAT and APOA1 were further validated using enzyme-linked immunosorbent assay (ELISA) (n = 120). It was found that AAT and APOA1 are significantly upregulated in OSCC and these glycoproteins are independent risk factors of OSCC. The clinical utility of AAT and APOA1 as potential biomarkers of OSCC is needed for further evaluation. © 2021, The Author(s), under exclusive licence to Springer Science+Business Media, LLC part of Springer Nature.

Item Type: Article
Uncontrolled Keywords: Glycoprotein; Glycoproteomics; Lectin; Oral squamous cell carcinoma
Subjects: R Medicine > RK Dentistry
Divisions: Faculty of Dentistry
Depositing User: Ms. Juhaida Abd Rahim
Date Deposited: 03 May 2021 05:59
Last Modified: 03 May 2021 05:59
URI: http://eprints.um.edu.my/id/eprint/25925

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