Graphene oxide-gold nanoparticle-aptamer complexed probe for detecting amyloid beta oligomer by ELISA-based immunoassay

Zhao, Jing and Chang, Wenlong and Liu, Lu and Xing, Xiaoming and Zhang, Chao and Meng, Huihong and Gopinath, Subash C.B. and Lakshmipriya, Thangavel and Chen, Yeng and Liu, Yonggang (2021) Graphene oxide-gold nanoparticle-aptamer complexed probe for detecting amyloid beta oligomer by ELISA-based immunoassay. Journal of Immunological Methods, 489. p. 112942. ISSN 0022-1759

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Official URL: https://doi.org/10.1016/j.jim.2020.112942

Abstract

Highly sensitive and easy detection method for Alzheimer's disease (AD) with a suitable biomarker is mandatory for preventing the factors resulting from AD. This research reports a modified ELISA with graphene for the detection of AD biomarker amyloid beta (Aβ) oligomer. Gold nanoparticle (AuNP) conjugated aptamer was used as the capture probe and attached on ELISA-graphene oxide surface through the amine linker. Antibody was used as the detection molecule to reach the maximum detection of Aβ oligomer. Suitable level of APTMS (2%), size of AuNP (30 nm) and aptamer concentration (2 μM) were optimized. This sandwich pattern of aptamer-Aβ oligomer-antibody helps to reach the detection at 50 pM on the optimized ELISA surface and the control experiments in the absence of Aβ oligomer or anti-Aβ oligomer antibody did not show the significant optical detection at 492 nm, indicting the specific detection. Further, Aβ oligomer spiked artificial cerebrospinal fluid did not interfere the detection of Aβ oligomer, confirming the selective detection. This new and modified ELISA surface helps to reach the lower detection of Aβ oligomer and diagnose AD. © 2020 Elsevier B.V.

Item Type: Article
Uncontrolled Keywords: Alzheimer's disease; Aptamer; Nanomaterial; Biomarker
Subjects: R Medicine
R Medicine > RK Dentistry
Divisions: Faculty of Dentistry
Depositing User: Ms. Juhaida Abd Rahim
Date Deposited: 21 Apr 2021 03:33
Last Modified: 21 Apr 2021 03:33
URI: http://eprints.um.edu.my/id/eprint/25878

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