Zen, L.P.Y. and Lai, Meng Yee and Lau, Yee Ling (2020) Elimination of contamination in loop-mediated isothermal amplification assay for detection of human malaria. Tropical Biomedicine, 37 (4). pp. 1124-1128. ISSN 2521-9855, DOI https://doi.org/10.47665/TB.37.4.1124.
Full text not available from this repository.Abstract
The LAMP assay, amplifies the target DNA rapidly, with 10-fold greater sensitivity than conventional PCR. The greater sensitivity also comes with greater risks of contamination. To overcome this issue, the current project includes either uracil DNA glycosylase (UDG) or a mineral oil overlay in the LAMP assay. Our results indicated that UDG or a mineral oil overlay can effectively prevent carryover contamination in the LAMP assay for the detection of human malaria. By incorporating these preventative methods, contamination can be eliminated and LAMP can potentially be used in the field; and point of care diagnosis for human malaria. © 2020, Malaysian Society for Parasitology. All rights reserved.
| Item Type: | Article |
|---|---|
| Funders: | Long Term Research Grant Scheme (LRGS), LR002D-2018 from the Ministry of Education, Malaysia |
| Uncontrolled Keywords: | Elimination; contamination; detection; human malaria |
| Subjects: | R Medicine |
| Divisions: | Faculty of Medicine |
| Depositing User: | Ms. Juhaida Abd Rahim |
| Date Deposited: | 08 Feb 2021 04:55 |
| Last Modified: | 08 Feb 2021 04:55 |
| URI: | http://eprints.um.edu.my/id/eprint/25711 |
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