A simple technique for chromosome preparation from embryonic tissues of teleosts for ploidy verification

Pradeep, P.J.; Srijaya, T.C.; Zain, R.B.; Papini, A.; Chatterji, A.K. (2011) A simple technique for chromosome preparation from embryonic tissues of teleosts for ploidy verification. Caryologia, 64 (2). pp. 235-241. ISSN 0008-7114

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      Abstract

      The modified preparation technique developed in the present study has provided an excellent chromosome metaphase spread from the whole body of larvae of the red hybrid tilapia Oreochromis mossambicus (Peters, 1852) X Oreochromis niloticus (Linnaeus, 1758). Unlike other chromosome preparation methods, this modified technique showed a reduction in cell loss while suspending cells during the dropping process. This modified technique can therefore be widely applied not only to various tilapias but also to other teleostean fishes for preparing chromosome spreads. The best treatment parameters for preparing good chromosome spreads from red hybrid tilapia were optimized as 0.01 concentration of colchicine for 4-6 hours, hypotonic 40 minutes treatment, fixation with carnoy solution at 3:1 ratio and a concentration of 10 Giemsa for 20 minutes.

      Item Type: Article
      Journal or Publication Title: Caryologia
      Additional Information: Pradeep, Padmaja Jayaprasad Srijaya, Thekkeparambil Chandrabose Zain, Rosnah Binti Mohd Papini, Alessio Chatterji, Anil Kumar
      Uncontrolled Keywords: Chromosome; Diploids, Hybrid Tilapia; Metaphase Spread; Triploids, Rainbow-Trout; Irideus Gibbons; Fish; Differentiation; Perciformes; Colchicine; Induction; Karyotype; Evolution; Triploidy
      Subjects: R Medicine > RK Dentistry > Oral surger
      Divisions: Faculty of Dentistry > Dept of Oral Pathology & Oral Medicine & Periodontology
      Depositing User: Prof. Dr. Rosnah Mohd Zain
      Date Deposited: 08 Feb 2012 10:25
      Last Modified: 11 Dec 2013 15:01
      URI: http://eprints.um.edu.my/id/eprint/2551

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