Ong, Nyee Huey and Chua, Chong Long and Liew, Jonathan Wee Kent and Wan Sulaiman, Wan Yusoff and Chan, Yoke Fun and Sam, I-Ching and Vythilingam, Indra (2020) Vector competence of Malaysian Aedes aegypti to Zika virus and impact of sequential arbovirus infections. Acta Tropica, 208. p. 105472. ISSN 0001-706X, DOI https://doi.org/10.1016/j.actatropica.2020.105472.
Full text not available from this repository.Abstract
Zika virus (ZIKV) is a mosquito-borne flavivirus with global impact since 2015. Although ZIKV was first isolated from Aedes aegypti in Malaysia in 1965, not much is known about the competency of Malaysian Ae. aegypti to ZIKV. To date only 9 cases of ZIKV have been reported in Malaysia despite the abundance of mosquito vectors. This study aimed to determine the susceptibility of Ae. aegypti to ZIKV, and the impact of sequential infections in Ae. aegypti mosquitoes with DENV serotype 2 (DENV-2) followed by ZIKV. Field-caught urban Ae. aegypti were orally challenged with a Martinique strain of ZIKV, and midgut, head/thorax and saliva were collected at 3, 7 and 14 days post-infection (dpi). At 14 dpi, ZIKV-exposed mosquitoes had infection and dissemination rates of 59% (n=10/17) and 90% (n=9/10), respectively. Average titres of 3.9 and 4.4 log pfu infectious ZIKV were recovered in midgut and head/thorax, respectively. In sequential infection, prior exposure of Ae. aegypti to DENV did not affect the subsequent ZIKV infection in head/thorax albeit with a low sample size. In conclusion, Malaysian urban Ae. aegypti is susceptible to the contemporary Asian lineage of ZIKV. The established and continuous DENV circulation in Ae. aegypti did not suppress ZIKV emergence in Malaysia. Other factors contributing to low level of ZIKV circulation in Malaysia remain to be explored. © 2020 Elsevier B.V.
| Item Type: | Article |
|---|---|
| Funders: | Ministry of Higher Education, Malaysia (FRGS FP016-2017A), Faculty research grant (GPF012C-2018) |
| Uncontrolled Keywords: | Aedes aegypti; Zika virus; Dengue serotype 2 virus; Sequential infection; Malaysia |
| Subjects: | R Medicine |
| Divisions: | Faculty of Medicine |
| Depositing User: | Ms. Juhaida Abd Rahim |
| Date Deposited: | 25 Aug 2020 06:59 |
| Last Modified: | 25 Aug 2020 06:59 |
| URI: | http://eprints.um.edu.my/id/eprint/25452 |
Actions (login required)
 |
View Item |
