Human telomerase reverse transcriptase expression in oral carcinogenesis - a preliminary report

Kumar, S.K.S.; Zain, R.B.; Ismail, S.M.; Cheong, S.C. (2005) Human telomerase reverse transcriptase expression in oral carcinogenesis - a preliminary report. Journal of Experimental and Clinical Cancer Research, 24 (4). pp. 639-646. ISSN 0392-9078

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      Abstract

      Human telomerase reverse transcriptase (hTERT), the catalytic subunit of telomerase, is strongly associated with telomerase activity implicated in cellular immortalization and tumorigenesis. In situ detection of hTERT will aid in determining the localization of telomerase positive cells. The aim of this study was to detect hTERT protein expression in multistep oral carcinogenesis using paraffin embedded tissue samples, and to study the relationship of hTERT expression with different histological stages in oral carcinogenesis. Normal (n=4), hyperplastic (n=4), dysplastic (n=4) and neoplastic (n=10) oral epithelia representing different histological stages in oral carcinogenesis were included in the study. hTERT protein detection was done by immunohistochemistry (IHC) technique. Nuclear staining intensities were noted and the hTERT-labelling index was determined. Dysplastic and neoplastic oral epithelia showed an increased percentage of hTERT positive cells (Grade 4: >50 positive staining nuclei) with intense staining in the basal, parabasal and superficial layers of the epithelia, unlike normal oral mucosa which showed intense staining only in the basal and parabasal cell layers, which are the normal proliferative progenitor compartments. hTERT protein expression was elevated with the corresponding advancement of the histological stages of oral carcinogenesis, from normal to hyperplasia to dysplasia to carcinoma. There seems to be an upregulation of hTERT protein expression during the progression of oral cancer, therefore, this may indicate the feasibility of IHC detection of hTERT protein in oral carcinogenesis as a potential diagnostic or prognostic marker.

      Item Type: Article
      Creators:
      1. Kumar, S.K.S.
      2. Zain, R.B.(Department of Oral Pathology and Oral Medicine and Periodontology, Faculty of Dentistry, University of Malaya)
      3. Ismail, S.M.
      4. Cheong, S.C.
      Journal or Publication Title: Journal of Experimental and Clinical Cancer Research
      Additional Information: Cited By (since 1996): 4 Export Date: 7 December 2011 Source: Scopus Times Cited: 3 Kumar, SKS Zain, RB Ismail, SM Cheong, SC Address: Kumar, S.K.S.a d , Zain, R.B.a , Ismail, S.M.b , Cheong, S.C.c a Dept. of Oral Pathology, Oral Medicine and Periodontology, Faculty of Dentistry, University of Malaya, Kuala Lumpur, Malaysia b Dept. of Oral and Maxillofacial Surgery, Faculty of Dentistry, University of Malaya, Kuala Lumpur, Malaysia c Cancer Research Initiatives Foundation (CARIF), Outpatient Centre, Subang Jaya Medical Centre, Subang Jaya, Selangor Darul Ehsan, Malaysia d Department of Oral Pathology, Oral Medicine and Periodontology, Faculty of Dentistry, University of Malaya, Kuala Lumpur 50603, Malaysia
      Uncontrolled Keywords: Human telomerase reverse transcriptase Immunohistochemistry Oral precancer Oral squamous cell carcinoma Telomerase
      Subjects: R Medicine > RK Dentistry > Oral surger
      Divisions: Faculty of Dentistry > Dept of Oral Pathology & Oral Medicine & Periodontology
      Depositing User: Prof. Dr. Rosnah Mohd Zain
      Date Deposited: 08 Feb 2012 09:37
      Last Modified: 11 Dec 2013 15:35
      URI: http://eprints.um.edu.my/id/eprint/2544

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