Poon, Nee Kiew and Teo, Chee How and Othman, Rofina Yasmin (2020) Differential gene expression analysis of Secreted in Xylem (SIX) genes from Fusarium oxysporum f. sp. cubense tropical race 4 in Musa acuminata cv. Berangan and potential application for early detection of infection. Journal of General Plant Pathology, 86 (1). pp. 13-23. ISSN 1345-2630, DOI https://doi.org/10.1007/s10327-019-00882-6.
Full text not available from this repository.Abstract
Homologues of Secreted in Xylem (SIX) genes, a group of known fungal effectors, were here identified in the genome of Fusarium oxysporum f. sp. cubense tropical race 4 (FocTR4). Expression of FocSIX genes was analyzed in FocTR4 vegetative mycelia and FocTR4-infected banana roots using quantitative reverse transcription PCR (qRT-PCR). Differential gene expression of all FocSIX genes identified in the genome was assessed over time in banana roots inoculated with FocTR4. Two groups of FocSIX genes expression pattern were found; Group I (FocSIX8a, FocSIX1b, FocSIX1c and FocSIX2) represents FocSIX genes that were only expressed in planta, and Group II consists of the remaining FocSIX genes (FocSIX1a, FocSIX6, FocSIX9 and FocSIX13) that were expressed in vitro and in planta. With the FocTR4 genomic information, Group I members can potentially serve as biomarkers for the early detection of Fusarium wilt infection in banana. © 2019, The Phytopathological Society of Japan and Springer Japan KK, part of Springer Nature.
| Item Type: | Article |
|---|---|
| Funders: | University of Malaya HIR-MOE (Cycle 3) Grant-UM.C/625/1/HIR/MOE/SC/14/4, Postgraduate Research Grant (PPP)-PG178-2016A, BKP078-2016, RU006-2017 |
| Uncontrolled Keywords: | Banana; Fusarium oxysporum f. sp. cubense; Fusarium wilt; Gene expression; Secreted in Xylem (SIX) gene |
| Subjects: | Q Science > Q Science (General) Q Science > QH Natural history S Agriculture > S Agriculture (General) |
| Divisions: | Faculty of Science > Institute of Biological Sciences Deputy Vice Chancellor (Research & Innovation) Office > Centre for Research in Biotechnology for Agriculture |
| Depositing User: | Ms. Juhaida Abd Rahim |
| Date Deposited: | 17 Aug 2020 06:34 |
| Last Modified: | 17 Aug 2020 06:34 |
| URI: | http://eprints.um.edu.my/id/eprint/25390 |
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