Roslan, Nurnadiah and Teh, Ser Huy and Lee, Yean Kee and Abd Rahman, Noorsaadah and Mohamed, Zulqarnain (2020) Analyses of boesenbergia rotunda cell suspension cultures with overexpressed chalcone synthase (CHS) manifesting changes on the expression level of flavonoid-related genes. Malaysian Journal of Science, 39 (1). pp. 15-29. ISSN 1394-3065, DOI https://doi.org/10.22452/mjs.vol39no1.2.
Full text not available from this repository.Abstract
Over the years, hundreds of potentially important and useful compounds from Boesenbergia rotunda (B. rotunda) have been successfully isolated and studied as these compounds have been shown to exhibit pharmacological and traditional medicinal properties in treating illness and diseases. In this study, a high-throughput large-scale RNA sequencing (RNA-Seq) was used to generate massive transcriptome sequence data on cell suspension cultures with overexpressed Chalcone synthase. These data were further analysed to understand the influence of CHS overexpression in cell suspension cultures of B. rotunda and further examine the regulation of genes associated with flavonoids accumulation. Based on the distribution of differentially expressed genes (DEGs), 305 up-regulated genes and 181 down-regulated genes which were consistently shown between wildtypes and transgenics. However, further comparative analysis against the KEGG database highlighted the fact that expression level of flavonoid-related genes were down-regulated, which is deviated from our assumption. Also, due to the overexpression of BrCHS2 transcript, unigenes that are responsible in the plant-pathogen interaction pathway also increased as a response towards stress. With a sufficient amount of data and transcripts obtained, identification of potential unigenes could be used for the biosynthesis of specific compounds such as flavonoids and plant defence metabolites in B. rotunda. Also, from these analyses, there is a potential biosynthesis pathway diverges from main pathways could be strongly supported and suggested. © 2020 Malaysian Abstracting and Indexing System. All rights reserved.
| Item Type: | Article |
|---|---|
| Funders: | University of Malaya through these research grants FL 002-2010 and PV085-2012 |
| Uncontrolled Keywords: | Boesenbergia rotunda; Differentially expressed genes; Flavonoids; Rhizomes; RNA-Seq |
| Subjects: | Q Science > Q Science (General) Q Science > QD Chemistry Q Science > QH Natural history |
| Divisions: | Faculty of Science > Department of Chemistry Faculty of Science > Institute of Biological Sciences |
| Depositing User: | Ms. Juhaida Abd Rahim |
| Date Deposited: | 05 Aug 2020 01:53 |
| Last Modified: | 05 Aug 2020 01:53 |
| URI: | http://eprints.um.edu.my/id/eprint/25243 |
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