Investigating intraspecific variation of Ralstonia solanacearum strains in West Malaysia using whole cell fatty acid analysis

Khakvar, Reza and Sijam, Kamaruzaman and Radu, Son and Thong, Kwai Lin and Wong, Y. (2007) Investigating intraspecific variation of Ralstonia solanacearum strains in West Malaysia using whole cell fatty acid analysis. In: 29th Symposium of the Malaysian Society for Microbiology, 24-26 November 2007, Kuala Terengganu, Terengganu, Malaysia. (Unpublished)

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Abstract

Surveys were conducted between the years of 2005-2006 at several locations in the northern, central and southern parts of West Malaysia to study the polymorphism of R. solanacearum strains. These sites included vegetable and farms with known hosts of the pathogen, such as banana, tomato, eggplant, chili and tobacco. Sample collection was conducted by randomly collecting the suspected plants and weeds, including soil and water samples, from selected areas. The bacterium was detected in all samples by Nested-PCR. The bacteria were isolated from positive samples by using semi-selective media and were further confirmed by biochemical and BIOLOG test. FAME (Fatty Acid Methyl Esters) profiling? was performed for the detection of polymorphism among bacterial isolates. The results showed that the fatty acid composition varies for all R. solanacearum isolates. Grouping of R. solanacearum isolates by fatty acid composition suggested the existence of eight groups namely group A, B, C, D, E, F, G andH.

Item Type: Conference or Workshop Item (Paper)
Additional Information: Conference paper
Uncontrolled Keywords: Ralstonia solanacearum; Farms; Vegetable; Bacteria; Biochemical
Subjects: Q Science > Q Science (General)
Q Science > QH Natural history
S Agriculture > S Agriculture (General)
Divisions: Faculty of Science > Institute of Biological Sciences
Depositing User: Mr. Mohd Safri
Date Deposited: 17 Jul 2020 01:03
Last Modified: 17 Jul 2020 01:03
URI: http://eprints.um.edu.my/id/eprint/25112

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