Bacterial diversity of decomposing oil palm empty fruit bunches based on PCR and denaturing gradient gel electrophoresis of 16S rRNA gene fragments

Goh, C.M. and Liew, P.W.Y. and Jong, B.C. and Thong, Kwai Lin (2007) Bacterial diversity of decomposing oil palm empty fruit bunches based on PCR and denaturing gradient gel electrophoresis of 16S rRNA gene fragments. In: 29th Symposium of the Malaysian Society for Microbiology, 24-26 November 2007, Kuala Terengganu, Terengganu, Malaysia. (Unpublished)

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Abstract

The microbial community that inhabit the thermophilic stage of composting process consist of complex thermophile communities that interact with each other to biodegrade lignocellulosic materials. The diversity of this community during oil palm empty fruit bunches (OPEFB) composting was investigated using PCR and denaturing gradient gel electrophoresis (DGGE) of 16S rRNA gene fragments. Compost samples were collected during the thermophilic phase with a temperature of between 60°C-62°C. Based on DGGE profile of 16S rDNA amplicons, four discrete bands were observed. Sequence analysis of the excised bands showed that sequences of these bands are closely related to those found in uncultured bacteria from other compost environments. The results suggest that the composting process of OPEFB during the thermophilic phase were dominated by yet-uncultured bacteria species.

Item Type: Conference or Workshop Item (Paper)
Additional Information: Conference paper
Uncontrolled Keywords: Thermophilic; Microbial species; rRNA gene; Ethidium bromide; Mutation
Subjects: Q Science > Q Science (General)
Q Science > QR Microbiology
Divisions: Faculty of Science > Institute of Biological Sciences
Depositing User: Mr. Mohd Safri
Date Deposited: 17 Jul 2020 01:06
Last Modified: 17 Jul 2020 01:06
URI: http://eprints.um.edu.my/id/eprint/25111

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