Multiplex, bead-based suspension array for the molecular determination of Salmonella H serogroups

Thong, Kwai Lin and McQuiston, John and Fiztergerald, Collette and Fields, Patrichia (2007) Multiplex, bead-based suspension array for the molecular determination of Salmonella H serogroups. In: 29th Symposium of the Malaysian Society for Microbiology, 24-26 November 2007, Kuala Terengganu, Terengganu, Malaysia. (Unpublished)

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Abstract

Salmonella enterica continues to be an important foodborne pathogen worldwide. Serotyping is the fundamental approach in the epidemiology of Salmonella. It consists of the immunologic classification of two surface structures, O-polysaccharide (0 antigen) and flagellin protein (H antigen). Salmonella serotypes are the basis for national surveillance networks and are critical to the identification of outbreaks.The current conventional serotyping procedure is time-consuming, requiring 3 to 5 days or more to fully detennine the serotype, thus delaying infonnation and costing laboratories in overhead. Furthennore, production and quality control of the hundreds of antisera required for serotyping are difficult and time consuming. To avoid the difficulties of antisera production and to take advantage of the automability of DNA technology, the National Centre for Infectious Diseases, CDC, Atlanta has developed a system for the determination of serotypes based on DNA markers within genes responsible for 0 and H antigen expression. This system combines PCR with a bead-based suspension array detection system. The objective of this paper is to describe the application of the Luminex® Multianalyte profling (xMAP) technology to detect the H antigens of Salmonella.

Item Type: Conference or Workshop Item (Paper)
Additional Information: Conference paper
Uncontrolled Keywords: Salmonella enterica; Antigen expression; DNA; Genes; Hybridization
Subjects: Q Science > Q Science (General)
Q Science > QR Microbiology
Divisions: Faculty of Science > Institute of Biological Sciences
Depositing User: Mr. Mohd Safri
Date Deposited: 17 Jul 2020 01:08
Last Modified: 17 Jul 2020 01:08
URI: http://eprints.um.edu.my/id/eprint/25110

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