Boo, Lily and Selvaratnam, Lakshmi and Cheh, Chin Tai and Ahmad, Tunku Sara and Kamarul, Tunku (2011) Expansion and preservation of multipotentiality of rabbit bone-marrow derived mesenchymal stem cells in dextran-based microcarrier spin culture. Journal of Materials Science: Materials in Medicine, 22 (5). pp. 1343-1356. ISSN 0957-4530, DOI https://doi.org/10.1007/s10856-011-4294-7.
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Abstract
The use of mesenchymal stem cells (MSCs) in tissue repair and regeneration despite their multipotentiality has been limited by their cell source quantity and decelerating proliferative yield efficiency. A study was thus undertaken to determine the feasibility of using microcarrier beads in spinner flask cultures for MSCs expansion and compared to that of conventional monolayer cultures and static microcarrier cultures. Isolation and characterization of bone marrow derived MSCs were conducted from six adult New Zealand white rabbits. Analysis of cell morphology on microcarriers and culture plates at different time points (D0, D3, D10, D14) during cell culture were performed using scanning electron microscopy and bright field microscopy. Cell proliferation rates and cell number were measured over a period of 14 days, respectively followed by post-expansion characterization. MTT proliferation assay demonstrated a 3.20 fold increase in cell proliferation rates in MSCs cultured on microcarriers in spinner flask as compared to monolayer cultures (p < 0.05). Cell counts at day 14 were higher in those seeded on stirred microcarrier cultures (6.24 ± 0.0420 cells/ml) × 10(5) as compared to monolayer cultures (0.22 ± 0.004 cells/ml) × 10(5) and static microcarrier cultures (0.20 ± 0.002 cells/ml) × 10(5). Scanning electron microscopy demonstrated an increase in cell colonization of the cells on the microcarriers in stirred cultures. Bead-expanded MSCs were successfully differentiated into osteogenic and chondrogenic lineages. This system offers an improved and efficient alternative for culturing MSCs with preservation to their phenotype and multipotentiality.
| Item Type: | Article |
|---|---|
| Funders: | UNSPECIFIED |
| Additional Information: | Tissue Engineering Group, Department of Orthopaedic Surgery, Faculty of Medicine, National Orthopaedic Centre of Excellence for Research and Learning, University of Malaya, 50603, Kuala Lumpur, Malaysia. boolily83@gmail.com |
| Uncontrolled Keywords: | Mesenchymal Stem Cell; Monolayer Culture; Spinner Flask; Chondrogenic Medium; Microcarrier Culture |
| Subjects: | R Medicine |
| Divisions: | Faculty of Medicine |
| Depositing User: | Nor Izzati Ab Razak |
| Date Deposited: | 26 Jan 2012 04:24 |
| Last Modified: | 14 Nov 2019 07:24 |
| URI: | http://eprints.um.edu.my/id/eprint/2509 |
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