Setiawan, Agus Budi and Teo, Chee How and Kikuchi, Shinji and Sassa, Hidenori and Kato, Kenji and Koba, Takato (2020) Centromeres of Cucumis melo L. comprise Cmcent and two novel repeats, CmSat162 and CmSat189. PLoS ONE, 15 (1). e0227578. ISSN 1932-6203, DOI https://doi.org/10.1371/journal.pone.0227578.
Full text not available from this repository.Abstract
Centromeres are prerequisite for accurate segregation and are landmarks of primary constrictions of metaphase chromosomes in eukaryotes. In melon, high-copy-number satellite DNAs (SatDNAs) were found at various chromosomal locations such as centromeric, pericentromeric, and subtelomeric regions. In the present study, utilizing the published draft genome sequence of melon, two new SatDNAs (CmSat162 and CmSat189) of melon were identified and their chromosomal distributions were confirmed using fluorescence in situ hybridization. DNA probes prepared from these SatDNAs were successfully hybridized to melon somatic and meiotic chromosomes. CmSat162 was located on 12 pairs of melon chromosomes and co-localized with the centromeric repeat, Cmcent, at the centromeric regions. In contrast, CmSat189 was found to be located not only on centromeric regions but also on specific regions of the chromosomes, allowing the characterization of individual chromosomes of melon. It was also shown that these SatDNAs were transcribed in melon. These results suggest that CmSat162 and CmSat189 might have some functions at the centromeric regions. © 2020 Setiawan et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
| Item Type: | Article |
|---|---|
| Funders: | Japan Society for Promotion of Science (JSPS) KAKENHI Grant Number JP 16K07588 |
| Uncontrolled Keywords: | Centromere; Kinetochore; Chromosome Segregation |
| Subjects: | S Agriculture > S Agriculture (General) |
| Divisions: | Deputy Vice Chancellor (Research & Innovation) Office > Centre for Research in Biotechnology for Agriculture |
| Depositing User: | Ms. Juhaida Abd Rahim |
| Date Deposited: | 24 Jun 2020 04:51 |
| Last Modified: | 24 Jun 2020 04:51 |
| URI: | http://eprints.um.edu.my/id/eprint/24979 |
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