TaqMan probe based multiplex quantitative PCR assay for determination of bovine, porcine and fish DNA in gelatin admixture, food products and dietary supplements

Sultana, Sharmin and Hossain, M.A. Motalib and Azlan, Azrina and Johan, Mohd Rafie and Chowdhury, Zaira Zaman and Ali, Md. Eaqub (2020) TaqMan probe based multiplex quantitative PCR assay for determination of bovine, porcine and fish DNA in gelatin admixture, food products and dietary supplements. Food Chemistry, 325. p. 126756. ISSN 0308-8146

Full text not available from this repository.
Official URL: https://doi.org/10.1016/j.foodchem.2020.126756

Abstract

Detection of animal materials in gelatin-based products is required to address religious and cultural concerns, because porcine and bovine gelatins are prohibited in Halal, Kosher and Hindus consumer goods. In this paper, multiplex quantitative polymerase chain reaction (qPCR) assay using TaqMan probe was developed to discriminate bovine, porcine and fish gelatin species in a single assay platform. The assay was specific to cattle, pigs and fish, having been tested against 14 non-target species. The limit of detection, under gelatin admixed conditions, was 0.005 ng/µL. Finally, a pilot survey was undertaken testing 35 Halal branded processed food and dietary items. Out of 35 samples, only two were found to be positive for porcine species. The authenticity of these two qPCR products was confirmed by DNA sequencing analysis, which showed 99–100% similarity with Sus scrofa (Wild boar) species. © 2020 Elsevier Ltd

Item Type: Article
Uncontrolled Keywords: Multiplex quantitative PCR; Internal amplification control; TaqMan Probes; PCR efficiency; Limit of quantification
Subjects: Q Science > QH Natural history
S Agriculture > S Agriculture (General)
Divisions: Deputy Vice Chancellor (Research & Innovation) Office > Centre for Research in Biotechnology for Agriculture
Deputy Vice Chancellor (Research & Innovation) Office > Nanotechnology & Catalysis Research Centre
Depositing User: Ms. Juhaida Abd Rahim
Date Deposited: 17 Jun 2020 00:57
Last Modified: 17 Jun 2020 00:57
URI: http://eprints.um.edu.my/id/eprint/24864

Actions (login required)

View Item View Item