Sultana, Sharmin and Hossain, M.A. Motalib and Azlan, Azrina and Johan, Mohd Rafie and Chowdhury, Zaira Zaman and Ali, Md. Eaqub (2020) TaqMan probe based multiplex quantitative PCR assay for determination of bovine, porcine and fish DNA in gelatin admixture, food products and dietary supplements. Food Chemistry, 325. p. 126756. ISSN 0308-8146, DOI https://doi.org/10.1016/j.foodchem.2020.126756.
Full text not available from this repository.Abstract
Detection of animal materials in gelatin-based products is required to address religious and cultural concerns, because porcine and bovine gelatins are prohibited in Halal, Kosher and Hindus consumer goods. In this paper, multiplex quantitative polymerase chain reaction (qPCR) assay using TaqMan probe was developed to discriminate bovine, porcine and fish gelatin species in a single assay platform. The assay was specific to cattle, pigs and fish, having been tested against 14 non-target species. The limit of detection, under gelatin admixed conditions, was 0.005 ng/µL. Finally, a pilot survey was undertaken testing 35 Halal branded processed food and dietary items. Out of 35 samples, only two were found to be positive for porcine species. The authenticity of these two qPCR products was confirmed by DNA sequencing analysis, which showed 99–100% similarity with Sus scrofa (Wild boar) species. © 2020 Elsevier Ltd
| Item Type: | Article |
|---|---|
| Funders: | University of Malaya Postgraduate Research Grant No. PG 251- 2016A |
| Uncontrolled Keywords: | Multiplex quantitative PCR; Internal amplification control; TaqMan Probes; PCR efficiency; Limit of quantification |
| Subjects: | Q Science > QH Natural history S Agriculture > S Agriculture (General) |
| Divisions: | Deputy Vice Chancellor (Research & Innovation) Office > Centre for Research in Biotechnology for Agriculture Deputy Vice Chancellor (Research & Innovation) Office > Nanotechnology & Catalysis Research Centre |
| Depositing User: | Ms. Juhaida Abd Rahim |
| Date Deposited: | 17 Jun 2020 00:57 |
| Last Modified: | 17 Jun 2020 00:57 |
| URI: | http://eprints.um.edu.my/id/eprint/24864 |
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