Tan, Irene Kit Ping and Foong, Choon Pin and Tan, Hua Tiang and Lim, Hui and Zain, Noor Afiqah Ahmad and Tan, Yung Chie and Hoh, Chee Choong and Sudesh, Kumar (2020) Polyhydroxyalkanoate (PHA) synthase genes and PHA-associated gene clusters in Pseudomonas spp. and Janthinobacterium spp. isolated from Antarctica. Journal of Biotechnology, 313. pp. 18-28. ISSN 0168-1656, DOI https://doi.org/10.1016/j.jbiotec.2020.03.006.
Full text not available from this repository.Abstract
The polyhydroxyalkanoate (PHA) producing capability of four bacterial strains isolated from Antarctica was reported in a previous study. This study analyzed the PHA synthase genes and the PHA-associated gene clusters from the two antarctic Pseudomonas isolates (UMAB-08 and UMAB-40) and the two antarctic Janthinobacterium isolates (UMAB-56 and UMAB-60) through whole-genome sequence analysis. The Pseudomonas isolates were found to carry PHA synthase genes which fall into two different PHA gene clusters, namely Class I and Class II, which are involved in the biosynthesis of short-chain-length-PHA (SCL-PHA) and medium-chain-length-PHA (MCL-PHA), respectively. On the other hand, the Janthinobacterium isolates carry a Class I and an uncharacterized putative PHA synthase genes. No other gene involved in PHA synthesis was detected in close proximity to the uncharacterized putative PHA synthase gene in the Janthinobacterium isolates, therefore it falls into a separate clade from the ordinary Class I, II, III and IV clades of PHA synthase (PhaC) phylogenetic tree. Multiple sequence alignment showed that the uncharacterized putative PHA synthase gene contains all the highly conserved amino acid residues and the proposed catalytic triad of PHA synthase. PHA biosynthesis and in vitro PhaC enzymatic assay results showed that this uncharacterized putative PHA synthase from Janthinobacterium sp. UMAB-60 is funtional. This report adds new knowledge to the PHA synthase database as we describe scarce information of PHA synthase genes and PHA-associated gene clusters from the antarctic bacterial isolates (extreme and geographically isolated environment) and comparing with those from non-antarctic PHA-producing bacteria. © 2020 Elsevier B.V.
| Item Type: | Article |
|---|---|
| Funders: | University of Malaya Research Grant ( UMRG RP007-2012B ), Research University Grant (RUI) from Universiti Sains Malaysia ( 1001/PBIOLOGI/8011060 ), MyBrainSc scholarship awarded by Ministry of Higher Education (Malaysia), Public Service Department of Malaysia scholarship scheme |
| Uncontrolled Keywords: | Antarctic bacteria; PHA operon; PHA synthase; Polyhydroxyalkanoates (PHA); Whole-genome sequencing |
| Subjects: | Q Science > Q Science (General) Q Science > QH Natural history |
| Divisions: | Faculty of Science > Institute of Biological Sciences |
| Depositing User: | Ms. Juhaida Abd Rahim |
| Date Deposited: | 16 Jun 2020 01:26 |
| Last Modified: | 16 Jun 2020 01:26 |
| URI: | http://eprints.um.edu.my/id/eprint/24835 |
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