Ong, L.Y.; Pang, T.; Lim, S.H.; Tan, E.L.; Puthucheary, S.D. (1989) A simple adherence test for detection of IgM antibodies in typhoid. Journal of medical microbiology, 29 (3). pp. 195-8. ISSN 0022-2615Full text not available from this repository.
A simple adherence test to detect IgM antibodies in patients with typhoid is described. The test utilises the IgM-"capture" approach, in which the test serum is applied to microtitration plate wells previously coated with anti-human IgM, followed by application of a stained Salmonella typhi antigen suspension which shows adherence in positive cases. By this test, 58 (95%) of 61 sera from confirmed cases of typhoid possessed IgM antibodies to the H or O or both antigens of S. typhi. In patients for whom a diagnosis of typhoid was based only on a significant Widal-test titre, 31 (41%) of 76 sera had IgM antibodies to the H or O or both antigens of S. typhi. Some cross-reactivity of the IgM antibodies was detected, especially with the O antigens of S. paratyphi A and B. A total of 82 sera from non-typhoidal fevers (leptospirosis, typhus, dengue fever) showed no reactivity in this test. In normal sera there was no detectable IgM to the O antigen of S. typhi and only a small number (3.9%) had low levels of IgM to the H antigen. The significance and potential importance of this simple, sensitive, specific and economical test is discussed.
|Journal or Publication Title:||Journal of medical microbiology|
|Uncontrolled Keywords:||IgM antibodies|
|Subjects:||R Medicine > R Medicine (General)|
|Divisions:||Faculty of Medicine|
|Depositing User:||Mr. Faizal Hamzah|
|Date Deposited:||01 Mar 2011 10:14|
|Last Modified:||21 Oct 2014 10:11|
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