A simple adherence test for detection of IgM antibodies in typhoid.

Ong, L Y; Pang, T; Lim, S H; Tan, E L; Puthucheary, S D (1989) A simple adherence test for detection of IgM antibodies in typhoid. Journal of medical microbiology, 29 (3). pp. 195-8. ISSN 0022-2615

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Abstract

A simple adherence test to detect IgM antibodies in patients with typhoid is described. The test utilises the IgM-"capture" approach, in which the test serum is applied to microtitration plate wells previously coated with anti-human IgM, followed by application of a stained Salmonella typhi antigen suspension which shows adherence in positive cases. By this test, 58 (95%) of 61 sera from confirmed cases of typhoid possessed IgM antibodies to the H or O or both antigens of S. typhi. In patients for whom a diagnosis of typhoid was based only on a significant Widal-test titre, 31 (41%) of 76 sera had IgM antibodies to the H or O or both antigens of S. typhi. Some cross-reactivity of the IgM antibodies was detected, especially with the O antigens of S. paratyphi A and B. A total of 82 sera from non-typhoidal fevers (leptospirosis, typhus, dengue fever) showed no reactivity in this test. In normal sera there was no detectable IgM to the O antigen of S. typhi and only a small number (3.9%) had low levels of IgM to the H antigen. The significance and potential importance of this simple, sensitive, specific and economical test is discussed.

Item Type: Article
Creators:
  1. Ong, L Y
  2. Pang, T
  3. Lim, S H
  4. Tan, E L
  5. Puthucheary, S D
Journal or Publication Title: Journal of medical microbiology
Uncontrolled Keywords: IgM antibodies
Subjects: R Medicine > R Medicine (General)
Divisions: Faculty of Medicine
Depositing User: Mr. Faizal Hamzah
Date Deposited: 01 Mar 2011 10:14
Last Modified: 01 Mar 2011 10:14
URI: http://eprints.um.edu.my/id/eprint/246

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