Lipoxygenase inhibiting activity of some Malaysian plants

Chung, L.Y.; Soo, W.K.; Chan, K.Y.; Mustafa, M.R.; Goh, S.H.; Imiyabir, Z. (2009) Lipoxygenase inhibiting activity of some Malaysian plants. Pharmaceutical Biology, 47 (12). pp. 1142-1148. ISSN 1388-0209

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    Abstract

    In this study 160 plant samples representing more than 30 plant families collected from the Malaysian forests were assessed for their ability to inhibit lipoxygenase activity. The lipoxygenase inhibiting activity was measured using the 96-well microplate-based ferric oxidation of xylenol orange (FOX) assay. The screening parameters, including z’ factor, indicated that the assay method adopted was robust and suitable for highthroughput screening. In the preliminary screen, four plant extracts displayed inhibitory activity of 70% or higher. The active plant extracts were isolated from Agelaea borneensis Merr. (Connaraceae) (bark) (IC 50,1.6 μg/mL), Chisocheton polyandrus Merr. (Meliaceae (bark) (3 μg/mL), Garcinia cuspidata King (Guttiferae) (bark) (28.3 μg/mL) and Timonius flavescens Baker (Rubiaceae) (leaf) (8.9 μg/mL).

    Item Type: Article
    Creators:
    1. Chung, L.Y.
    2. Soo, W.K.
    3. Chan, K.Y.
    4. Mustafa, M.R.
    5. Goh, S.H.
    6. Imiyabir, Z.
    Journal or Publication Title: Pharmaceutical Biology
    Additional Information: Address for Correspondence: L. Y. Chung, Department of Pharmacy, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia. Tel.: +603 79674971; Fax: +603 79674964; E-mail: chungly@um.edu.my
    Uncontrolled Keywords: Anti-inflammatory; FOX assay; Inflammation; Lipoxygenase; Malaysian Plants
    Subjects: R Medicine
    S Agriculture > S Agriculture (General)
    Divisions: Faculty of Medicine
    Depositing User: Ms Haslinda Lahuddin
    Date Deposited: 17 Jan 2012 12:33
    Last Modified: 11 Dec 2013 12:10
    URI: http://eprints.um.edu.my/id/eprint/2455

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